重组猪α干扰素对猪繁殖与呼吸综合征(PRRS)阳性血清中和猪繁殖与呼吸综合征病毒(PRRSV)影响的研究  

Effect of Recombinant Porcine α-interferon on Neutralization of PRRS Virus in PRRS Positive Serum

作　　者：魏波 丛雁方 祝俊鹏 刘晓婧 蒋贻海 WEI Bo;CONG Yanfang;ZHU Junpeng;LIU Xiaojing;JIANG Yihai(Qingdao Weilan Biological Co.Ltd.,Qingdao Shandong 266114,China)

机构地区：[1]青岛蔚蓝生物股份有限公司,山东青岛266114

出　　处：《畜牧兽医科学（电子版）》2022年第11期7-9,共3页Graziery Veterinary Sciences：Electronic Version

基　　金：猪重大疫病快速检测与疫情预警体系构建及新型标记疫苗创制(2019JZZY010720);青岛市科技惠民示范引导专项(20-3-4-21-nsh)。

摘　　要：采用猪繁殖与呼吸综合征病毒(PRRSV)/Marc-145细胞体系验证重组猪α干扰素(rPoIFN-α)增强PRRSV阳性血清对PRRSV的中和作用。结果表明,PRRSV阳性血清稀释至1∶0.588时,加上20 000 U/mL rPoIFN-α作用后,可以中和10~4TCID个猪繁殖与呼吸综合征病毒。研究结果在体外细胞水平明确rPoIFN-α增强了PRRSV阳性血清对PRRSV的中和作用,为进一步将rPoIFN-α应用于PRRSV的净化提供参考。Porcine reproductive and respiratory syndrome virus(PRRSV)/Marc-145 cell system was used to verify that recombinant porcine interferon α(rPoIFN-α) enhanced neutralization of PRRSV positive serum against PRRSV.The results showed that when the PRRSV positive serum was diluted to 1∶0.588 and 20 000 U/mL rPoIFN-α was added,104TCID50 blue ear viruses could be neutralized.Our results confirmed that rPoIFN-α enhanced the neutralization effect of PRRSV positive serum on PRRSV in vitro,providing a reference for further application of rPoIFN-α in the purification of PRRSV.

关 键 词：重组猪Α干扰素 增强中和作用 猪繁殖与呼吸综合征 

分 类 号：S858.28[农业科学—临床兽医学]