纳米羟基磷灰石/阿司匹林/聚乙烯醇/明胶/海藻酸钠水凝胶支架的制备与表征  

Preparation and characterization of nano-hydroxyapatite/aspirin/polyvinyl alcohol/gelatin/sodium alginate hydrogel scaffolds

作　　者：李绍萍 杨雨晴 萧文云登 尹璐璐 刘溧博 刘莹 孙一凡 陈志宇[1] Li Shaoping;Yang Yuqing;Xiao Wenyundeng;Yin Lulu;Liu Libo;Liu Ying;Sun Yifan;Chen Zhiyu(Department of Prosthodontics,School of Stomatology·Hospital of Stomatology,Hebei Medical University,Hebei Provincial Key Laboratory of Stomatology,Hebei Provincial Clinical Research Center for Oral Diseases,Shijiazhuang 050017,Hebei Province,China;School of Stomatology,Hebei Medical University,Shijiazhuang 050017,Hebei Province,China)

机构地区：[1]河北医科大学口腔医学院·口腔医院修复科,河北省口腔医学重点实验室,河北省口腔疾病临床医学研究中心,河北省石家庄市050017 [2]河北医科大学口腔医学院,河北省石家庄市050017

出　　处：《中国组织工程研究》2023年第25期3956-3963,共8页Chinese Journal of Tissue Engineering Research

基　　金：河北省“三三三人才工程”资助项目(A202102010),项目负责人:陈志宇;河北省政府资助临床医学优秀人才培养项目:(361029),项目负责人:陈志宇。

摘　　要：背景:阿司匹林是经典非类固醇抗炎药物,合适剂量的阿司匹林可调节免疫、促进成骨。制备出可缓释阿司匹林、调控骨缺损区免疫微环境和加速骨缺损修复的骨组织工程材料是目前的研究热点。目的:制备可调控免疫微环境和加速骨缺损修复的新型水凝胶支架。方法:制备含有质量分数0%,10%和20%纳米羟基磷灰石的纳米羟基磷灰石/阿司匹林/聚乙烯醇/明胶/海藻酸钠水凝胶支架,对水凝胶支架的微观结构、孔隙率、化学成分、晶相结构、药物缓释性能、力学性能、溶胀性能和降解性能进行表征。通过细胞增殖和细胞毒性实验评价水凝胶支架的生物相容性。结果与结论:①扫描电镜和孔隙率检测结果表明,0%,10%纳米羟基磷灰石组水凝胶支架具有更仿生的层级多孔结构、孔隙通联性和孔隙率;②傅里叶红外光谱和X射线衍射结果表明,水凝胶支架内各原料以物理、化学双交联方式结合,且不会破坏纳米羟基磷灰石的晶相结构;③力学性能结果表明,10%纳米羟基磷灰石组水凝胶支架具有最佳的压缩模量和压缩强度;④药物释放性能结果显示,随着纳米羟基磷灰石质量的增加,水凝胶支架的阿司匹林累计释放率降低,但是药物突释率降低、缓释时间延长;⑤溶胀性能和降解性能结果表明,随着纳米羟基磷灰石质量的增加,水凝胶支架的溶胀率和降解率下降;⑥采用水凝胶支架浸提液培养小鼠前成骨细胞,细胞增殖和细胞毒性实验结果表明,水凝胶支架浸提液可促进细胞增殖,无细胞毒性;⑦结果表明,10%纳米羟基磷灰石组水凝胶支架具有更佳仿生的表征和生物相容性,有望作为骨组织工程支架进行成骨和调节免疫潜力的进一步研究。BACKGROUND:Aspirin is a classic non-steroidal anti-inflammatory drug.Appropriate doses of aspirin can regulate immunity and promote osteogenesis.The preparation of bone tissue engineering materials,which can release aspirin,regulate the immune microenvironment of the bone defect area and accelerate the repair of the bone defect,and is a current research focus.OBJECTIVE:To prepare a novel hydrogel scaffold that can modulate the immune microenvironment and quickly repair the bone defect area.METHODS:Hydrogel scaffolds containing 0%,10%and 20%of nano-hydroxyapatite/aspirin/polyvinyl alcohol/gelatin/sodium alginate were prepared.The microstructure,porosity,chemical composition,crystal structure,drug release properties,mechanical properties,swelling properties and degradation properties of the hydrogel scaffolds were characterized.The biocompatibility of the hydrogel scaffolds was evaluated by cell proliferation and cytotoxicity experiments.RESULTS AND CONCLUSION:(1)Scanning electron microscope and porosity results showed that hydrogel scaffolds in the 0%nano-hydroxyapatite group and 10%nano-hydroxyapatite group had better biomimetic hierarchical porous structure,pore connectivity,and porosity.(2)The results of Fourier transform infrared spectroscopy and X-ray diffraction showed that the raw materials in the hydrogel scaffold were combined by physical and chemical double crosslinking,and the crystal phase structure of nano-hydroxyapatite was not be destroyed.(3)The results of mechanical properties showed that the 10%nanohydroxyapatite group had the best compressive modulus and compressive strength.(4)The results of drug release properties showed that the cumulative release rate of aspirin decreased with the increase of nano-hydroxyapatite,but the drug burst release rate decreased and the sustained release time prolonged.(5)The results of swelling properties and degradation properties showed that with the increase of nano-hydroxyapatite,the swelling rate and degradation rate of hydrogel scaffolds of each group decrease

关 键 词：双交联 纳米羟基磷灰石 水凝胶支架 阿司匹林 免疫 缓释 组织工程 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学] R783.1