纳米羟基磷灰石封闭牙本质小管的效果  

Sealing effect of nano hydroxyapatite on dentinal tubules

作　　者：于蓝宁 王倩 靳友士 费晓文 王青山[1] Yu Lanning;Wang Qian;Jin Youshi;Fei Xiaowen;Wang Qingshan(Binzhou Medical University Hospital,Binzhou 256603,Shandong Province,China;Shangdi Branch of Ruitai Stomatological Hospital,Beijing100085,China)

机构地区：[1]滨州医学院附属医院,山东省滨州市256603 [2]瑞泰口腔医院上地分院,北京市100085

出　　处：《中国组织工程研究》2023年第25期3964-3970,共7页Chinese Journal of Tissue Engineering Research

基　　金：山东省自然科学基金项目(ZR2018LH010),项目负责人:王青山。

摘　　要：背景:纳米羟基磷灰石是优良的生物活性材料,对脱矿牙釉质和牙本质具有一定的保护作用,但其溶液对脱矿牙本质再矿化的作用及其机制尚不明确。目的:探讨纳米羟基磷灰石用于防治牙本质敏感症和牙本质龋的可行性。方法:收集废弃的人双尖牙标本,制备出72份牙本质薄片,分6组处理:A组脱矿后浸入10%纳米羟基磷灰石溶液中,B组脱矿后浸入6%生物活性玻璃溶液中,C组脱矿后浸入10%纳米羟基磷灰石与6%生物活性玻璃复合溶液中,D组脱矿后浸入0.05%NaF溶液中,E组脱矿后浸入人工唾液中,F组脱矿后浸入去离子水中,每组12个样本。处理14 d后,扫描电镜下观察样本工作面,利用能谱分析仪检测样本表面成分,激光共聚焦显微镜下检测牙本质罗丹明B染色情况。结果与结论:①扫描电镜观察:A组牙本质小管口被完全封闭,牙本质表面被一层紧密连接的颗粒物均匀覆盖;C组牙本质小管部分封闭;B、D、E组牙本质表面及牙本质小管内见少量散在颗粒物,牙本质小管口呈开放状;F组牙本质表面及牙本质小管内未见颗粒物沉积,牙本质小管口呈开放状态;②X射线能谱分析:A组Ca/P比为1.61,最接近健康牙本质理论值,Ca/P比高于B、E、F组(P<0.05),与C组Ca/P比无差异(P>0.05);③激光共聚焦显微镜检测:A组荧光强度和荧光面积最小,平均荧光强度小于其他5组(P<0.05);C组平均荧光强度小于B、D、E、F组(P<0.05);④结果表明:纳米羟基磷灰石对脱矿牙本质具有良好的再矿化作用,纳米羟基磷灰石与生物活性玻璃联合应用亦能沉积在牙本质表面,但并无增效作用,封闭效果不及单独使用纳米羟基磷灰石。BACKGROUND:Nano hydroxyapatite is an excellent bioactive material,which has a certain protective effect on demineralized enamel and dentin,but the effect and mechanism of its solution on remineralization of demineralized dentin are not clear.OBJECTIVE:To explore the feasibility of nano hydroxyapatite in the prevention and treatment of dentin sensitivity and dentin caries.METHODS:Abandoned human bicuspid specimens were collected,and 72 dentin slice samples were prepared from isolated bicuspids.The samples were randomly divided into 6 groups,with 12 samples per group:After demineralization,each group was immersed in 10%nano hydroxyapatite solution(group A),6%bioactive glass solution(group B),10%nano hydroxyapatite and 6%bioactive glass composite solution(group C),0.05%sodium fluoride(group D),artificial saliva(group E),and deionized water(group F).After 14 days of treatment,working surfaces of samples were observed by scanning electron microscope.The surface composition was detected with energy spectrum analyzer.Dentin Rhodamine B staining was detected under a confocal laser microscope.RESULTS AND CONCLUSION:(1)Scanning electron microscopy:The dentinal tubules orifice in group A was completely sealed,and the surface of the dentin was uniformly covered by a layer of tightly connected granular material.The dentinal tubules in group C were partially sealed.A small amount of scattered particles were found on the dentin surface and in the dentinal tubules of group B,group D and group E,and the dentinal tubules were open.In group F,there was no particulate matter deposition on the dentin surface or in the dentinal tubules,and the dentinal tubules were open.(2)X-ray energy dispersive spectrocopy results:The Ca/P ratio of group A was 1.61,which was closest to the theoretical value of healthy dentin;the Ca/P ratio was higher in group A than that in the groups B,E,and F(P<0.05).There was no significant difference in Ca/P ratio between group A and group C(P>0.05).(3)Laser confocal microscopy test results showed that the fluor

关 键 词：牙本质 纳米羟基磷灰石 生物活性玻璃 脱矿 再矿化 牙本质小管 

分 类 号：R459.9[医药卫生—治疗学] R-331[医药卫生—临床医学] R318.08