丝素胶原蛋白复合支架联合富血小板血浆修复皮肤损伤  

Silk fibroin collagen composite scaffold combined with platelet-rich plasma for repairing skin injury

作　　者：刘继超[1] 赵金龙[1] 于洋[1] Liu Jichao;Zhao Jinlong;Yu Yang(The 3201 Hospital Affiliated to Xi’an Jiaotong University School of Medicine,Hanzhong 723000,Shaanxi Province,China)

机构地区：[1]西安交通大学医学院附属三二〇一医院,陕西省汉中市723000

出　　处：《中国组织工程研究》2023年第25期3971-3976,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:胶原与丝素蛋白复合构建的组织工程支架,在皮肤、神经、血管、骨、软骨等组织工程领域应用广泛。富血小板血浆是血液经过2次离心获得的血小板浓缩物,含有多种组织修复需要的生长因子,可促进组织再生与创面愈合。目的:观察丝素胶原蛋白支架复合富血小板血浆在皮肤创面愈合中的作用。方法:分别制备丝素胶原蛋白支架、SD大鼠富血小板血浆。取8周龄SD大鼠48只,每只背部制作2个直径2 cm的全层皮肤缺损创面,分4组处理:空白组缺损处注射生理盐水,单纯支架组缺损处植入丝素胶原蛋白复合支架,富血小板血浆组创缘注射同种异体富血小板血浆,联合组缺损处植入丝素胶原蛋白复合支架+创缘注射同种异体富血小板血浆,每组12只。造模后检测创面愈合率、创面炎症因子水平、创面组织学观察及相关蛋白表达。结果与结论:①联合组造模后第7,14天的创面愈合率大于空白组、单纯支架组、富血小板血浆组(P<0.05)。②联合组造模后第7,14天的肿瘤坏死因子α、白细胞介素6水平均低于空白组、单纯支架组、富血小板血浆组(P<0.05)。③造模后第14天的苏木精-伊红及Masson染色显示,空白组缺损处仅见少量的新生毛细血管与混乱排列的胶原纤维组织;其他3组可见大量的新生毛细血管与腺体样组织,胶原纤维排列较规律,其中以联合组新生血管最多、腺体样组织层次更清晰、胶原纤维排列更规则。免疫组化染色显示,空白组、单纯支架组、富血小板血浆组CD31+细胞密度少于联合组(P<0.05)。④Western blot检测显示,相较于空白组、单纯支架组、富血小板血浆组,联合组创面Ⅰ型胶原、Ⅲ型胶原、基质金属蛋白酶抑制剂1的蛋白表达升高(P<0.05),基质金属蛋白酶3、基质金属蛋白酶9蛋白表达降低(P<0.05)。⑤结果表明,丝素胶原蛋白支架复合富血小板血浆可通过抑制炎症反�BACKGROUND:Tissue enginee ring scaffolds constructed by collagen and silk fibroin are widely used in tissue engineering fields such as skin,nerves,blood vessels,bone,and cartilage.Platelet-rich plasma is a platelet concentrate obtained by centrifuging blood twice.It contains a variety of growth factors required for tissue re pair and can promote tissue regeneration and wound healing.OBJECTIVE:To observe the effect of silk fibroin collagen scaffold combined with platelet-rich plasma on skin wound healing.METHODS:Silk fibroin collagen scaffolds and SD rat platelet-rich plasma were prepared separately.Totally 488-week-old SD rats were selected,and 2 fullthickness skin defect wounds with a diameter of 2 cm were made on the back of each rat.They were randomly divided into four groups(n=12).In the blank gro up,normal saline was injected into the defect site.Silk fibroin-collagen composite scaffolds were implanted in the defects of the simple scaffold group.The platelet-rich plasma group was injected with allogeneic platelet-rich plasma at the wound margin.In the combined group,silk fibroin-collagen composite scaffolds were implanted in the defect site and allogeneic platelet-rich plasma was injected into the wound margin.Wound healing rate,wound inflammatory factor level,wound histological morphology and related protein expression were detected after model establishment.RESULTS AND CONCLUSION:(1)The wound healing rate of the combined group on days 7 and 14 after modeling was higher than that of the blank group,the simple scaffold group,and the platelet-rich plasma group(P<0.05).(2)The levels of tumor necrosis factorαand interleukin-6 in the combined group on days7 and 14 days after modeling were lower than those in the blank group,the simple scaffold group,and the platelet-rich plasma group(P<0.05).(3)On day 14 after modeling,hematoxylin-eosin staining and Masson staining showed that only a few new capillaries and disordered collagen fiber tissue were seen at the defect site of the blank group.A large number of new ca

关 键 词：丝素胶原蛋白复合支架 富血小板血浆 全层皮肤缺损 皮肤损伤 创面 创面愈合 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学] R622.1