载抗菌肽壳聚糖/骨粉/纳米微晶纤维素颌骨修复支架的理化性能  

Physicochemical properties of chitosan/bone powder/cellulose nanocrystals scaffold loaded with antimicrobial peptides for jaw repair

作　　者：于爱敏 徐婷 朱韵莹 梁坚强 武东辉 Yu Aimin;Xu Ting;Zhu Yunying;Liang Jianqiang;Wu Donghui(Stomatology Hospital of Haizhu district,Guangzhou 510405,Guangdong Province,China)

机构地区：[1]广州市海珠区口腔医院,广东省广州市510405

出　　处：《中国组织工程研究》2023年第25期3999-4005,共7页Chinese Journal of Tissue Engineering Research

基　　金：广州市海珠区区科技项目(2018-38);项目负责人:武东辉。

摘　　要：背景:单一生物材料较难达到理想骨组织工程支架材料的标准,多种生物材料复合及优势互补是构建出理想支架的有效途径。目的:构建出新型颌骨修复支架,并检测其关键理化性能。方法:采用冷冻干燥法制备羧甲基壳聚糖/骨粉/纳米微晶纤维素/抗菌肽颌骨修复支架,观察分析支架的微观结构,测定支架的抗拉强度、断裂伸长率,评估支架的吸水率、体外降解率、热稳定性和抗菌肽缓释作用。结果与结论:①扫描电镜下可见,制备的颌骨修复支架具有均匀多孔网状结构,平均孔径为114.9μm,支架的吸水率为(698.8±53.8)%,支架的断裂伸长率介于8%-10%,抗拉强度介于0.7-0.9 MPa,应力-应变关系与自然的软骨组织相类似;②热重曲线显示,在200℃以下,颌骨修复支架材料轻微失重;在200-400℃之间,支架材料明显失重;在600℃以上时,支架材料失重严重;③体外药物释放结果显示,该支架在最初的12 h内释放出(58.40±1.79)%的抗菌肽,12 h后药物释放率下降,近20%的抗菌肽在6 d后仍未释放,有利于药效持续稳定;④将支架分别浸泡于PBS与含溶菌酶的PBS中,在28 d时,无溶菌酶与含溶菌酶条件下的支架降解率分别为70%,85%;⑤结果显示,载抗菌肽的羧甲基壳聚糖/骨粉/纳米微晶纤维素支架具有良好的吸水性能、热稳定性、降解性能与机械性能。BACKGROUND:It is difficult for a single biomaterial to reach the standard of an ideal scaffold material for bone tissue enginee ring.It is an effective way to construct an ideal scaffold by compounding and complementi ng the advantages of multi ple biomaterials.OBJECTIVE:To construct a new type of mandibular scaffold,and test the key physicochemical properties of the scaffold.METHODS:Antimicrobial peptide-loaded carboxymethyl chitosan/bone powder/cellulose nanocrystals scaffold was prepared by freeze-drying method.The microstructure of the scaffold was observed and analyzed,and the tensile strength and elongation at break of the scaffold were measured.The water absorption,degradation rate in vitro,thermal stability and release of antimicrobial peptide of the scaffold were evaluated.RESULTS AND CONCLUSION:(1)Scanning electron microscope showed that the scaffolds had a uniform porous network structure with an average pore size of 114.9μm.The water absorption rate of the scaffold was 698.8±53.8%.The fracture elongation of the scaffold was 8%-10%.The tensile strength was 0.7-0.9 MPa,and the stress-strain relationship was similar to that of natural ca rtilage tissue.(2)Thermogravimetric curve displayed that the weight loss of the stent was very slight below 200 a C,obvious between 200-400℃,and severe above 600℃.(3)The results of drug release in vitro showed that(58.40±1.79)%of antibacterial peptide was released from the scaffold within the first 12 hours,and the drug release rate decreased after 12 hours,and nearly 20%of antibacterial peptide was not released after 6 days,which was beneficial to the sustained and stable drug effect.(4)The scaffolds were separately soaked in PBS and PBS containing lysozyme.At 28 days,the degradation rates of scaffolds without lysozyme and with lysozyme were 70%and 85%,respectively.(5)These res ults exhibited that the carboxymethyl chitosan/bone powde r/cellulose nanoc rystals scaffold had good water absorption performance,thermal stability,degradation performance and mechanical

关 键 词：羧甲基壳聚糖 纳米微晶纤维素 羟基磷灰石 抗菌肽 颌骨 支架 骨组织工程 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R-331