茶黄素-3,3’-双没食子酸酯缓解人工假体磨损颗粒引起的成骨抑制  

Theaflavin-3,3’-digallate alleviates osteogenesis inhibition induced by wear particles in artificial prostheses

作　　者：侯振扬 孙义玲 苏长征 李振 许正文 李文明 柏家祥 Hou Zhenyang;Sun Yiling;Su Changzheng;Li Zhen;Xu Zhengwen;Li Wenming;Bai Jiaxiang(Department of Joint Sports Medicine,The Affiliated Tengzhou Hospital of Xuzhou Medical University(Tengzhou Central People’s Hospital),Tengzhou 277500,Shandong Province,China;Department of Tumor,The Affiliated Tengzhou Hospital of Xuzhou Medical University(Tengzhou Central People’s Hospital),Tengzhou 277500,Shandong Province,China;Department of Orthopedics,First Affiliated Hospital of Suzhou University,Suzhou 215006,Jiangsu Province,China)

机构地区：[1]徐州医科大学附属滕州医院(滕州市中心人民医院)关节运动医学科,山东省滕州市277500 [2]徐州医科大学附属滕州医院(滕州市中心人民医院)肿瘤内科,山东省滕州市277500 [3]苏州大学附属第一医院骨科,江苏省苏州市215006

出　　处：《中国组织工程研究》2023年第25期4069-4074,共6页Chinese Journal of Tissue Engineering Research

基　　金：徐州医科大学附属医院科技发展优秀人才基金项目(XYFY2020012),项目负责人:侯振扬。

摘　　要：背景:茶黄素-3,3’-双没食子酸酯通过抑制破骨细胞形成在骨质疏松动物模型中显示出良好的治疗效果,但其对成骨细胞作用的研究还不多见。目的:探讨茶黄素-3,3’-双没食子酸酯对人工假体周围骨溶解的作用。方法:①体外实验:选取生长状态良好的MC3T3-E1细胞,分4组培养:对照组加入成骨诱导分化培养基,钛颗粒组加入钛颗粒+成骨诱导分化培养基,低剂量组加入钛颗粒+茶黄素-3,3’-双没食子酸酯(1 mg/L)+成骨诱导分化培养基,高剂量组加入钛颗粒+茶黄素-3,3’-双没食子酸酯(10 mg/L)+成骨诱导分化培养基。成骨诱导培养2周后,进行碱性磷酸酶染色;成骨诱导3周后,进行茜素红染色。②体内实验:将32只C57BL/6J小鼠按随机数字表法分4组,空白对照组不进行任何处理,模型组将无菌钛颗粒均匀置于颅骨表面建立骨溶解模型,低剂量组、高剂量组分别将1,10 mg/(kg·d)的茶黄素-3,3’-双没食子酸酯经腹腔注射至骨溶解模型小鼠体内。给药2周后,进行颅骨micro-CT扫描。结果与结论:①体外实验:碱性磷酸酶与茜素红染色显示,钛颗粒可抑制MC3T3-E1细胞的碱性磷酸酶活性与矿化结节形成,茶黄素-3,3’-双没食子酸酯可抑制钛颗粒的作用,且具有剂量依赖性;②体内实验:micro-CT扫描显示,钛颗粒可引发颅骨周围骨溶解,高剂量茶黄素-3,3’-双没食子酸酯可抑制钛颗粒引起的骨溶解;③结果表明:茶黄素-3,3’-双没食子酸酯可促进成骨分化,抑制人工假体周围骨溶解。BACKGROUND:Theaflavin-3,3’-digallate has shown a good therapeutic effect in animal models of osteoporosis by inhibiting osteoclast formation,but there are few studies on osteogenesis.OBJECTIVE:To investigate the effect of theaflavin-3,3’-digallate on periprosthetic osteolysis.METHODS:(1)In vitro test:The MC3T3-E1 cells with good growth status were selected and cultured in four groups.The control group was added with osteogenic differentiation medium;the titanium particle group was added with titanium particles+osteogenic differentiation medium;the low-dose group was added with titanium particles+theaflavin-3,3’-digallate(1 mg/L)+osteogenic differentiation medium;high-dose group was added with titanium particles+theaflavin-3,3’-digallate(10 mg/L)+osteogenic differentiation medium.After 2 weeks of osteogenic induction,alkaline phosphatase staining was performed;after 3 weeks of osteogenic induction,alizarin red staining was performed.(2)In vivo experiment:32 C57BL/6J mice were randomly divided into 4 groups.The blank control group did not receive any treatment.In the model group,sterile titanium particles were evenly placed on the surface of the skull to establish an osteolysis model.The low-dose group and high-dose group were injected intraperitoneally with 1,10 mg/(kg·d)theaflavin-3,3’-digallate into osteolysis model mice.The mouse skull was analyzed by micro-CT 2 weeks after operation.RESULTS AND CONCLUSION:(1)In vitro experiments:alkaline phosphatase and alizarin red staining showed that titanium particles could inhibit alkaline phosphatase activity and mineralized nodule formation in MC3T3-E1 cells,and theaflavins-3,3’-bisgallate could inhibit the effect of titanium particles and was dose-dependent.(2)In vivo experiments:micro-CT scans showed that titanium particles could induce osteolysis around the skull,and high-dose theaflavin-3,3’-digallate could inhibit the osteolysis caused by titanium particles.(3)The results showed that theaflavin-3,3’-digallate could promote the osteogenic differenti

关 键 词：茶黄素-3 3’-双没食子酸酯 钛颗粒 成骨分化 假体周围骨溶解 假体松动 

分 类 号：R459.9[医药卫生—治疗学] R336[医药卫生—临床医学] R684