Bcl-xl 基因与颅内感染伴癫痫发作的关系研究  

Relationship between Bcl-xl gene and intracranial infection companying seizures

作　　者：陈镇 黄兴刚 杨帝琼 CHEN Zhen;HUANG Xinggang;YANG Diqiong(Department of Neurology,the First People's Hospital of Zunyi(The Third Affiliated Hospital of Zunyi Medical University),Zunyi(563000),Guizhou,China)

机构地区：[1]遵义市第一人民医院(遵义医科大学第三附属医院)神经内科,贵州遵义563000

出　　处：《癫痫与神经电生理学杂志》2022年第6期359-364,共6页Journal of Epileptology and Electroneurophysiology(China)

基　　金：遵义市科技和大数据局项目[遵市科合HZ字(2019)138号]。

摘　　要：目的探讨Bcl-xl基因与颅内感染伴癫痫发作的关系及其临床意义。方法选取2019年10月至2020年10月在遵义医科大学第三附属医院神经内科住院治疗的诊断为颅内感染伴癫痫发作的患者15例(观察组)与不伴癫痫发作的患者21例(对照组),收集并对比分析两组患者的基线资料及辅助检查结果(脑脊液、血常规、影像学及脑电图等),采用RT-PCR技术检测脑脊液中Bcl-xl mRNA的表达。结果观察组15例患者的年龄为14~53岁,诊断为病毒性脑炎或脑膜脑炎,其中9例的首发症状为全面强直阵挛发作(60%),1例为发作性行为异常(7%),5例为头昏头痛(33%)。对照组患者的年龄为14~67岁,诊断为病毒性脑炎或脑膜脑炎19例、结核性脑膜炎2例,其中19例的首发症状为头昏头痛(90%),1例为精神行为异常(5%),1例为眩晕(5%)。观察组患者脑电图异常6例,对照组患者脑电图异常8例。观察组中4例患者为颅内高压,3例患者的腰穿脑脊液常规提示总细胞数异常;对照组中4例患者为颅内高压,11例存在白细胞数异常,6例生化结果提示蛋白有不同程度的增高,2例生化结果提示糖氯下降及ADA升高。观察组患者脑脊液中Bcl-xl mRNA表达量(0.28±0.09)比对照组患者的表达量(0.80±0.12)有一定下降,差异有统计学意义(P<0.05)。结论Bcl-xl抗凋亡分子在颅内感染伴癫痫发作患者中表达下调,说明颅内感染伴癫痫发作患者的发病机制可能与细胞凋亡相关。Objective To investigate the relationship between Bcl-xl and intracranial infection companying seizures and its clinical significance.Methods From October 2019 to October 2020,the patients with intracranial infection diagnosed in the department of Neurology of the third affiliated hospital of Zunyi Medical University were divided into observation group(n=15,seizures)and control group(n=21,without seizures).Baseline data and auxiliary examination results(cerebrospinal fluid,blood routine examination,imaging and electroencephalography(EEG))of two groups were documented and compared.Bcl-xl mRNA expression level in cerebrospinal fluid was detected by RT-PCR.Results In observation group,the age ranged from 14 to 53 years old,and 15 patients were diagnosed as viral encephalitis or meningoencephalitis.There were 9 patients with generalized tonic clonic seizures(60%),1 patient with paroxysmal behavior abnormality(7%)and 5 patients with dizziness and headache(33%)as the first symptom.In control group,the age ranged from 14 to 67 years old.There were 19 patients diagnosed as viral encephalitis or meningoencephalitis,2 patients as tuberculous meningitis;19 patients with dizziness and headache(90%),1 patient with psychobehavioral abnormality(5%)as the first symptom,and 1 patient with vertigo(5%)as the first symptom.There were 6 patients with abnormal ambulatory eleroencephalography(AEEG)in observation group,8 patients with abnormal AEEG in control group.In observation group,4 patients had intracranial hypertension and 3 patients had abnormal total cell count in cerebrospinal fluid via lumbar puncture.In control group,4 patients had intracranial hypertension,11 patients with abnormal total cell count in cerebrospinal fluid via lumbar puncture,6 patients with increased proteins at different degrees revealed by biochemical test,2 patients with decreased sugar and chloride levels as well as increased adenosine deaminase(ADA).Bcl-xl mRNA expression level in cerebrospinal fluid of patients was lower in observation group than contr

关 键 词：颅内感染 癫痫 癫痫发作 BCL-XL 凋亡 

分 类 号：R51[医药卫生—内科学] R742.1[医药卫生—临床医学]