基于UPLC-Q-TOF-MS/MS技术的彝族药姜味草化学成分分析  

Analysis of Chemical Constituents in Yi Medicine Micromeria biflora(Buch.-Ham.exD.Don)Benth.by UPLC-Q-TOF/MS

作　　者：王梦[1,2,3] 田伟 甄亚钦[1,2,3] 吴玲芳 王迎春[1,2,3] 牛丽颖 WANG Meng;TIAN Wei;ZHEN Ya-qin;WU Ling-fang;WANG Ying-chun;NIU Li-ying(Hebei University of Chinese Medicine,Shijiazhuang 050091,China;Hebei TCM Formula Granule Innovation Center,Shijiazhuang 050091,China;Hebei TCM Quality Evaluation and Standardization Engineering Research Center,Shijiazhuang 050091,China)

机构地区：[1]河北中医学院,石家庄050091 [2]河北省中药配方颗粒技术创新中心,石家庄050091 [3]中药材品质评价与标准化河北省工程研究中心,石家庄050091

出　　处：《中国药学杂志》2022年第20期1717-1725,共9页Chinese Pharmaceutical Journal

基　　金：中央引导地方科技发展资金项目资助(206Z2501G);河北省重点研发计划项目资助(20372502D);河北省自然科学基金项目资助(H2019423050);河北省中医药管理局项目资助(2022363)。

摘　　要：目的应用超高效液相色谱-串联四级杆飞行时间质谱联用技术(UPLC-Q-TOF-MS/MS)对彝族药姜味草中的化学成分进行定性分析。方法Waters Acquity UPLC BEH C_(18)色谱柱(2.1 mm×100 mm,1.7μm),以乙腈(A)-体积比0.1%甲酸水溶液(B)梯度洗脱,质谱分析采用信息关联模式(IDA),在正、负离子模式下采集数据。结果根据化合物的精确分子质量和碎片离子信息,结合对照品比对和文献数据,从姜味草中鉴定推断出77个化学成分,包括黄酮类27个、有机酸类21个、苯丙素类8个、三萜酸类8个、氨基酸类6个,其他类:蒽醌类、环烯醚萜类、醇类等7个。结论首次对姜味草中的化学成分进行快速鉴别,为其质量控制及药效物质基础提供了科学依据。OBJECTIVE To analyze and identify the chemical constituents from Yi medicine Micromeria biflora(Buch.-Ham.exD.Don)Benth.by using UPLC-Q-TOF/MS.METHODS The separation was performed on Waters Acquity UPLC BEH C_(18)(2.1 mm×100 mm,1.7μm)column with gradient elution of acetonitrile(A)-0.1%formic acid(B).The MS analysis was based on information associated mode(IDA),and positive and negative ions were collected respectively.RESULTS Fragments were analyzed and identified according to the precise mass charge ratio of the compounds,secondary mass spectrometry,and comparison of reference materials and literature data.A total of 77 compounds were identified,including 27 flavonoids,21 organic acids,8 phenylpropanoids,8 triterpenoids,6 amino acids,7 anthraquinones,iridoids and alcohols.CONCLUSION The chemical components of Micromeria biflora(Buch-Ham.ex D.Don)Benth.are rapidly identified for the first time,which lays a foundation for the research of quality control and pharmacodynamic material basis.

关 键 词：彝族药 姜味草 高效液相色谱-电喷雾电离高分辨飞行时间质谱 裂解规律 黄酮 有机酸 苯丙素 氨基酸 

分 类 号：R284.1[医药卫生—中药学]