黄芪甲苷对心衰模型大鼠心肌炎性因子和MAPK信号通路的影响  

Effects of Astragaloside on Myocardial Inflammatory Factor and Gene Expression of MAPK Signaling Pathway in Rats with Heart Failure

作　　者：张欣媛[1] 熊鑫[1] 韩金霞[1] 刘静静[1] 王金文[1] 宫浩 ZHANG Xinyuan;XIONG Xin;HAN Jinxia;LIU Jingjing;WANG Jinwen;GONG Hao(Daqing Oilfield General Hospital,Daqing 163316,China)

机构地区：[1]大庆油田总医院,黑龙江大庆163316

出　　处：《中医药学报》2023年第1期30-35,共6页Acta Chinese Medicine and Pharmacology

基　　金：黑龙江省博士后基金资助项目(LBH-Z18249)。

摘　　要：目的:探究黄芪甲苷对心衰模型大鼠心肌炎性因子和MAPK信号通路的影响,以期为黄芪甲苷在心衰治疗中的应用提供参考。方法:雄性健康SD大鼠,采用异丙肾上腺素诱导法建立心衰模型,造模成功的80只大鼠称体质量后,随机分为模型组、黄芪甲苷高剂量组(3.5 mg/kg)、黄芪甲苷中剂量组(2.0 mg/kg)和黄芪甲苷低剂量组(0.5 mg/kg),每组20只。另选取20只健康大鼠作为对照组,对照组和模型组给予相同体积的生理盐水,共灌胃治疗40 d。观察大鼠一般状态,并对心脏功能、心肌炎性因子、MAPK信号通路相关蛋白含量及细胞凋亡相关因子基因表达进行检测。结果:灌胃黄芪甲苷能降低心衰模型大鼠的死亡数量,缓解体质量降低情况(P<0.05),心率和射血分数明显提高(P<0.05),心肌中TNF-α、IL-1β和IL-6含量明显降低(P<0.05),MAPK信号通路的P38、Erk和JNK蛋白含量明显降低(P<0.05),细胞凋亡相关因子Caspase-3、Bax基因表达降低(P<0.05),而细胞凋亡抑制基因Bcl-2表达增加(P<0.05),并且这种效果与剂量呈正相关。结论:黄芪甲苷能通过抑制MAPK信号通路蛋白表达进而抑制心衰模型大鼠心肌炎性因子的分泌及其介导的心肌细胞凋亡,提高心脏功能,降低心衰模型大鼠的死亡率和失重。Objective: To explore the effect of astragaloside on myocardial inflammatory factors and MAPK signaling pathway in treatment of heart failure(HF), aiming to provide the application reference of astragaloside in treating HF. Methods: Healthy male SD rats were established HF model by Isoprenaline. 80 rats with successful modeling were randomly divided into the model group, the high-dose astragaloside group(3.5 mg/kg), the medium-dose astragaloside group(2.0 mg/kg) and the low-dose astragaloside group(0.5 mg/kg), with 20 rats in each group. Another 20 healthy rats were taken as the blank control. After 40 d of administration, the general state and cardiac function of rats were detected. The expressions of myocardial inflammatory factors, the protein contents and apoptosis related genes of MAPK signaling pathway were detected. Results: Astragaloside could reduce the number of deaths and significantly alleviate the loss of body mass(P<0.05), significantly increase heart rate and ejection fraction(P<0.05), significantly decrease the contents of TNF-a, IL-1β and IL-6 in myocardium(P<0.05), significantly decrease the expressions of P38, Erk and JNK in MAPK signaling pathway(P<0.05), significantly decrease the gene expressions of Caspase-3 and Bax(P<0.05), and significantly increase the expression of Bcl-2 in the rats with HF(P<0.05);which showed positive dose-effect correlation. Conclusion: Astragaloside can inhibit inflammatory factors and cardiomyocyte apoptosis of HF by reducing protein expressions of MAPK signaling pathway, it can improve heart function, and reduce the mortality and weightlessness of the rats with HF.

关 键 词：黄芪甲苷 心衰 炎性因子 MPAK信号通路 

分 类 号：R285.5[医药卫生—中药学]