富氢液对大鼠心脏停搏心肺复苏后海马NLRP3炎症小体表达的影响  

Effects of hydrogen-rich fluid on NLRP3 inflammasome expression in the hippocampus of rats with cardiac arrest after cardiopulmonary resuscitation

作　　者：陈泳伊 马丽丽[1] 顾连兵[1] 卞清明[1] 惠康丽[2] 段满林[2] CHEN Yongyi;MA Lili;GU Lianbing;BIAN Qingming;HUI Kangli;DUAN Manlin(Department of Anesthesiology,Jiangsu Cancer Hospital,Jiangsu Institute of Cancer Research,Nanjing Medical University Cancer Hospital,Nanjing,Jiangsu 210009,China;Department of Anesthesiology,Nanjing General Hospital,School of Medicine,Nanjing University,Nanjing,Jiangsu 210002)

机构地区：[1]江苏省肿瘤医院麻醉科,江苏省肿瘤防治研究所麻醉科,南京医科大学附属肿瘤医院麻醉科,江苏南京210009 [2]南京大学医学院南京总医院麻醉科,江苏南京210002

出　　处：《徐州医科大学学报》2022年第11期794-798,共5页Journal of Xuzhou Medical University

基　　金：国家自然科学基金(81671884);江苏省肿瘤医院科研项目(ZM201806)。

摘　　要：目的评价富氢液对大鼠心搏骤停/心肺复苏大鼠脑损伤海马NOD样受体热蛋白结构域相关蛋白3(NOD-like receptor thermal protein domain associated protein 3,NLRP3)炎症小体表达的影响。方法清洁级雄性SD大鼠80只,体重280~350 g,8~10周龄,采用随机数字表法分为3组:假手术组(Sham组,n=20)、心脏停搏心肺复苏组(I/R组,n=30)和富氢液组(H组,n=30)。采用经食管电刺激法建立心脏停搏心肺复苏模型;Sham组大鼠只进行气管插管及动静脉穿刺,于自主循环恢复(ROSC)即刻及6 h腹腔内注射富氢液5 ml/kg;其余2组腹腔内注射等容量的生理盐水。于ROSC后48 h行神经功能评分(NDS);H-E染色观察CA1区并计数锥体细胞;采用Western blot法检测海马NLRP3、半胱天冬酶-1前体(pro-caspase-1)和凋亡相关斑点样蛋白(apoptosis-associated speck-like protein containing a CARD,ASC)的表达。结果与Sham组比较,I/R组和H组ROSC 48 h后神经功能评分降低(P<0.05),锥体细胞计数减少(P<0.05),海马NLRP3、pro caspase-1和ASC表达上调(P<0.05);与I/R组比较,H组神经功能评分升高(P<0.05),锥体细胞计数增多(P<0.05),海马NLRP3、pro caspase-1和ASC表达下调(P<0.05)。结论富氢液减轻心搏骤停/心肺复苏大鼠脑损伤的机制可能与抑制NLRP3炎症小体激活、减轻炎症反应有关。Objective To evaluate the effects of hydrogen-rich liquid on the expression of NOD-like receptor thermoprotein domain-associated protein 3(NLRP3)inflammsome in the hippocampus of rats with cerebral injury after cardiac arrest/cardiopulmonary resuscitation(CPR).Methods A total of 80 healthy male SD rats weighing 280-350 g and aged 8-10 weeks were selected.According to the random number table method,they were divided into three groups:a sham operation(Sham)group(n=20),a cardiopulmonary resuscitation(I/R)group(n=30)and a hydrogen-rich liquid(H)group(n=30).A cardiopulmonary resuscitation model of cardiac arrest was established through transesophageal electrical stimulation.Group S was subjected to endotracheal intubation and arteriovenous puncture alone,followed by intraperitoneal injection of 5 ml/kg hydrogen-rich solution immediately after recovery of spontaneous circulation(ROSC)and 6 h after ROSC.The other two groups were intraperitoneally injected with an equal volume of normal saline.Their neurological function score(NDS)was evaluated 48 h after ROSC.The CA1 area and pyramidal cell count were observed by H-E staining.The expression of NLRP3,pro-caspase-1 and apoptosis-associated speck-like protein containinga caspase recruitment domain(ASC)in the hippocampus were detected by Western blot.Results Compared with the Sham group,the I/R and H groups showed decreases in the neurological function score and pyramidal cell count 48 h after ROSC(P<0.05),with up-regulated expression of NLRP3,pro-caspase-1 and ASC in the hippocampus(P<0.05).Compared with the I/R group,the H group presented increases in neurological function score(P<0.05)and pyramidal cells count(P<0.05),as well as down-regulated expression of NLRP3,pro-caspase-1 and ASC(P<0.05).Conclusions Hydrogen-rich fluid can alleviate brain injury in rats with cardiac arrest/cardiopulmonary resuscitation,which may be related to inhibiting the activation of NLRP3 inflammsomeand alleviating inflammatory response.

关 键 词：氢 心脏停搏 心肺复苏术 再灌注损伤 NOD样受体热蛋白结构域相关蛋白3 海马 

分 类 号：R605.975[医药卫生—急诊医学]