不同时间甘精胰岛素干预对烧伤延迟复苏大鼠脏器氧化应激的影响  

Effects of insulin glargine at different times on organ oxidative stress in burned rats with delayed resuscitation

作　　者：赵晨 文雪 冯珺琳 李伟人 吴玓瓅 Zhao Chen;Wen Xue;Feng Junlin;Li Weiren;Wu Dili(Department of Burns and Plastic Surgery,Guizhou Medical University Affiliated Hospital,Guiyang 550004,China;Department of Burns and Plastic Surgery,Guizhou Provincial People′s Hospital,Guiyang 550002,China)

机构地区：[1]贵州医科大学附属医院烧伤整形外科,贵阳550004 [2]贵州省人民医院烧伤整形外科,贵阳550002

出　　处：《中华医学杂志》2022年第43期3476-3481,共6页National Medical Journal of China

基　　金：贵州省科技合作项目(LH-2015-7408)。

摘　　要：目的探讨不同时间低剂量甘精胰岛素干预在烧伤延迟复苏大鼠中的抗氧化效应,明确烧伤延迟复苏时较好的抗氧化干预时机。方法将50只雄性SD大鼠按随机数字表法分为假伤组、延迟复苏组、烧伤后即刻甘精胰岛素治疗组(即刻治疗组)、烧伤后2 h甘精胰岛素治疗组(2 h治疗组)、烧伤后6 h甘精胰岛素治疗组(6 h治疗组)等5组,每组各10只。甘精胰岛素各治疗组分别于烧伤后即刻、2 h、6 h皮下注射甘精胰岛素(1.0 U·kg^(-1)·d^(-1)),延迟复苏组于伤后6 h皮下注射等量生理盐水。延迟复苏组及各治疗组均于伤后6 h腹腔注射生理盐水(40 ml/kg)模拟液体延迟复苏;假伤组不给药且不进行液体复苏。假伤组于伤后即刻、其余各组于伤后24 h分别采集大鼠腹主动脉血及心脏、肾脏组织。分光光度法测定各组大鼠血糖及心肌和肾脏组织中铜锌超氧化物歧化酶(CuZn-SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、总抗氧化能力(T-AOC)、黄嘌呤氧化酶(XOD)和髓过氧化物酶(MPO)的活性,分析抗氧化干预的时机。结果与假伤组相比,延迟复苏组血糖升高[(10.72±0.80)比(6.57±0.82)mmol/L,P<0.001];心肌、肾脏组织中CuZn-SOD、CAT和GSH-Px的活性及T-AOC均降低(均P<0.05);XOD、MPO的活性均升高(均P<0.05)。与延迟复苏组相比,即刻、2 h和6 h治疗组血糖均降低(均P<0.05);即刻和2 h治疗组心肌、肾脏组织中CuZn-SOD、CAT和GSH-Px的活性及T-AOC均增高(均P<0.05);6 h治疗组仅心肌组织中GSH-Px、肾脏组织中CAT和GSH-Px的活性增高(均P<0.05)。与延迟复苏组相比,即刻治疗组心肌组织中MPO及XOD、肾脏组织中XOD的活性降低(均P<0.05);2 h治疗组心肌、肾脏组织中的MPO、XOD的活性均降低(均P<0.05);6 h治疗组心肌组织中MPO、肾脏组织中XOD的活性均降低(均P<0.05)。与即刻治疗组相比,2 h治疗组心肌组织中GSH-Px的活性升高(P<0.05),肾脏组织中CuZn-SOD、CAT和Objective To examine the antioxidant effect of low dosage insulin glargine intervention at different time in rats with delayed resuscitation after burn,in order to acquire a better time of antioxidant intervention during delayed resuscitation following burn injury.Methods With 10 rats in each group,50 male SD rats were assigned to sham injury group,delayed resuscitation group,immediate post-burn insulin glargine treatment group(immediate treatment group),2 hours post-burn insulin glargine treatment group(2 h treatment group),and 6 hours post-burn insulin treatment group(6 h treatment group)with random number table.Each treatment group received subcutaneous injections of insulin glargine(1.0 U·kg^(-1)·d^(-1))immediately,two hours and six hours after the burn,while the delayed resuscitation group received the same amount of normal saline six hours after the burn.To imitate delayed fluid resuscitation,the delayed resuscitation group and each therapy group were intraperitoneally injected with normal saline(40 ml/kg)6 hours after injury.No medicine and fluid resuscitation was administered to the sham injury group.Rats in the sham injury group had their abdominal aortic blood,hearts,and kidney tissues collected immediately after injury,while rats in the other groups had their blood and tissues collected 24 hours later.To analyze the timing of antioxidant intervention,the activities of CuZn-superoxide dismutase(CuZn-SOD),catalase(CAT),glutathione peroxidase(GSH-Px),total antioxidant capacity(T-AOC),xanthine oxidase(XOD)and myeloperoxidase(MPO)in blood glucose and myocardial and renal tissues were measured by spectrophotometry.Results Compared with the sham group,blood glucose levels in the delayed resuscitation group increased[(10.72±0.80)vs(6.57±0.82)mmol/L,P<0.001],while in the myocardium and kidney,the activities of CuZn-SOD,CAT,GSH-Px and T-AOC decreased(all P<0.05)and the activities of XOD and MPO increased(all P<0.05).Compared with the delayed resuscitation group,blood glucose decreased in the immediate,2 h,an

关 键 词：烧伤 甘精胰岛素 延迟复苏 血糖 氧化应激 大鼠 干预时机 

分 类 号：R644[医药卫生—外科学]