玉米杂交种农科大8号及其亲本指纹图谱构建  

Construction of Maize Hybrid Nongkeda No.8 and Its Parents’Fingerprint

作　　者：南文华[1] 吴权明[1] 张瑞平 王蕊[2] 孙佩[2] 张培风 赵彦峰 李合顺[2] 王学军[2] 刘文静 NAN Wen-hua;WU Quan-ming;ZHANG Rui-ping(Northwest A&F University,Xianyang,Shaanxi 712100;Xinxiang Academy of Agricultural Sciences,Xinxiang,Henan 453000)

机构地区：[1]西北农林科技大学,陕西咸阳712100 [2]新乡市农业科学院,河南新乡453000 [3]陕西省种子工作总站,陕西西安710000

出　　处：《安徽农业科学》2022年第23期81-83,共3页Journal of Anhui Agricultural Sciences

基　　金：陕西省科技统筹创新工程计划项目“玉米优异种质创制及新品种培育”(2011KTZB02-01-02);陕西省重点产业链玉米种业关键技术研究与应用项目“突破性玉米新品种选育”(2017ZDCXL-NY-02-03);陕西省科技厅重点研发计划“高产宜机收玉米种质资源创新及新品种选育”(2022NY-185)。

摘　　要：采用SSR分子标记技术,利用40对玉米核心引物对玉米杂交种农科大8号及其亲本进行分析,从中筛选出8对扩增产物条带清晰稳定,且亲本条带互补的引物用来构建农科大8号及其亲本的指纹图谱,这8对引物分别是phi053k2,bnlg2291k4,bnlg2305k4,umc1147y4,bnlg1520k1,umc1489y3,bnlg490y4,umc1936k4,并将指纹图谱数字化,计算出现相同指纹图谱的概率为7.63×10^(-6)。结果表明:出现相同指纹图谱的概率极低,证明筛选出的这8对引物可用于玉米杂交种农科大8号的真实性鉴定。We used SSR molecular marker technology to construct fingerprints of maize hybrid Nongkeda No.8 and its parents by screening 8 primers with clear bands and complement each other from 40 pairs of core primers.The 8 pairs of primers screened were phi053k2,bnlg2291k4,bnlg2305k4,umc1147y4,bnlg1520k1,umc1489y3,bnlg490y4,umc1936k4.And we digitized the fingerprint of Nongkeda No.8 to calculate the probability of the same fingerprint,which was 7.63×10^(-6).This explained that it was feasible to identify the authenticity of Nongkeda No.8 by the fingerprint which was constructed by the 8 pairs of primers.

关 键 词：玉米 农科大8号 SSR 指纹图谱 

分 类 号：S513[农业科学—作物学]