二裂酵母发酵产物溶胞物在化妆品中的功效评估  

Efficacy evaluation of bifida ferment lysate in cosmetics

作　　者：杨娟 刘新琳 郑雅莉 陈伟阶 邓小锋 陶宏兵 谢小保 李良秋 YANG Juan;LIU Xin-lin;ZHENG Ya-li;CHEN Wei-jie;DENG Xiao-feng;TAO Hong-bing;XIE Xiao-bao;LI Liang-qiu(Guangdong Demay New Materials Technology Co.,Ltd.,Zhaoqing,Guangdong 526238,China;Guangdong Institute of Microbiology,Guangzhou,Guangdong 510070,China;Guangdong Demay Biological Technology Co.,Ltd.,Guangzhou,Guangdong 510000,China)

机构地区：[1]广东迪美新材料科技有限公司,广东肇庆526238 [2]广东省科学院微生物研究所,广东广州510070 [3]广东迪美生物技术有限公司,广东广州510000

出　　处：《日用化学品科学》2022年第12期24-26,39,共4页Detergent & Cosmetics

基　　金：肇庆市第三批西江创新团队项目(肇人才办【2019】5号)。

摘　　要：通过构建细胞光损伤后修复模型,评估人永生化角质形成细胞(HaCaT)及人皮肤成纤维细胞(HDF)在UVB和UVA下辐照下二裂酵母发酵产物溶胞物Ferment-DFL抗光损伤、抗皱功效。实验表明,人体细胞受到光损伤时,5%和2%浓度的Ferment-DFL能显著提高细胞活性水平,降低细胞内ROS活性氧含量,促进I型胶原蛋白分泌;同时采用斑马鱼尾鳍切尾再生实验评估FermentDFL修复功效,其在6%浓度下,48 h后斑马鱼胚胎尾鳍修护促进率达到15%,能显著促进斑马鱼胚胎尾鳍再生,具有促进修护效果。By constructing a cell repair model after photodamage,the resistance to photodamage and the anti-wrinkle effect of bifida ferment lysate(Ferment-DFL)were evaluated using the human immortalized keratinocytes(HaCaT)and human skin fibroblasts(HDF)under UVB and UVA irradiation.The experimental data show that,when human cells are damaged by light,Ferment-DFL at concentrations of 5%and 2%can significantly increase the level of cell viability,reduce the content of reactive oxygen species(ROS)in cells and promote the secretion of type I collagen.The repair effect of Ferment-DFL was evaluated by regeneration experiment.At a concentration of 6%,after 48 hours,the repair-promoting rate for the tail fin of zebrafish embryo reaches 15%,indicating that it can significantly promote the regeneration of tail fin in zebrafish embryo.

关 键 词：二裂酵母发酵产物溶胞物 化妆品功效 细胞模型 斑马鱼模型 

分 类 号：TQ658[化学工程—精细化工]