河南省牛肠道病毒的遗传变异分析  

Genetic variation of bovine enterovirus strains from Henan Province

作　　者：常晓冉 林倩 钱明珠 胡俊英[1] 李欣 古丽巴哈尔·图尔荪 王新平 CHANG Xiaoran;LIN Qian;QIAN Mingzhu;HU Junying;LI Xin;GULBAHAR·Tursun;WANG Xinping(Collegeof VeterinaryMedicine,Jilin University,Changchun 130062,China;Henan Vocational College of Agriculture,Zhengzhou 451450,China;Key Laboratory of Zoonosis Research,Ministry of Education,Institute for Zoonosis,Jilin University,Changchun 130062,China)

机构地区：[1]吉林大学动物医学学院,吉林长春130062 [2]河南农业职业学院,河南郑州451450 [3]吉林大学人兽共患病研究所教育部人兽共患病重点实验室,吉林长春130062

出　　处：《中国兽医学报》2022年第10期1982-1987,2000,共7页Chinese Journal of Veterinary Science

基　　金：“十三五”国家重点研发计划资助项目(2017YFD0500104,2016YFD0500904);国家自然科学基金资助项目(31572531)。

摘　　要：为了解河南省不同地区牛肠道病毒(BEV)的遗传变异与病毒株间的分子差异,本研究应用PCR方法从河南省不同地区牛场确定为BEV的阳性样品或病毒分离株中扩增了病毒VP1基因并对其进行了序列测定和分析,结果显示检测出的11株BEV中,8株属于E种肠道病毒(EV-E),3株属于F种肠道病毒(EV-F)。VP1基因核苷酸序列的比对分析结果表明,8株不同EV-E毒株间的VP1同源性为70.4%~98.6%,它们与本实验室分离的国内首株EV-E HY12毒株的同源性为69.3%~80.3%;EV-F HeN-A12和HeN-YR91毒株与国内首株EV-F毒株BHM26的同源性为68.6%和70.8%,而与首株山羊EV-F毒株SD-S67的同源性为88.8%和73.7%。进一步分析显示,8株EV-E毒株可以进一步分类为2个基因型EV-E2和EV-E3;而3株EV-F毒株也可进一步分类为2个基因型EV-F1和EV-F4。结果表明,河南省不同地区牛群存在EV-E和EV-F感染和肠道病毒感染的多样性以及病毒株的分子差异,该研究为该病的防控提供流行病学理论依据。To understand the genetic variation and molecular differences of bovine enterovirus(BEV) strains isolated from different areas in Henan Province, PCR was employed to amplify the VP1 gene from BEV-positive specimen or isolates identified by a double antibody sandwich ELISA.Sequence analysis of PCR-amplified VP1 genes identified 11 BEV isolates.Out of 11 BEV strains, 8 strains were clustered to enterovirus E species(EV-E),while 3 strains were grouped to enterovirus F species.Sequence alignment analysis showed that the EV-E strains shared 69.3%-80.3% VP1 sequence homology with the first EV-E HY12 strain in China.The homology of VP1 sequence among different EV-E isolates ranged from 70.4% to 98.6%.Out of the three EV-F isolates, HeN-A12 and HeN-YR91 strains shared a 68.6% and 70.8% VP1 sequence with the EV-F BMH26 strain, while they had a 66.2% and 88.8% sequence homology with the first Caprine EV-F virus strain SD-S67.Phylogenetic analysis further grouped the eight EV-E strains into EV-E2 and EV-E3 genotypes, and three EV-F strains to EV-F1 and EV-F4 genotypes.In summary, we revealed the epidemiological background of BEV infection, the genetic variation on BEV strains, and the genotypes of EV-E and EV-F virus in Henan Province, which provided the basis for future prevention and control of this infection.

关 键 词：E种肠道病毒 F种肠道病毒 VP1 遗传变异 基因型 

分 类 号：S852.65[农业科学—基础兽医学]