Rapid identification of stigmastane-type steroid saponins from Vernonia amygdalina leaf based onα-glucosidase inhibiting activity and molecular networking  

作　　者：GAO Juanjuan ZHAO Mengling SHAN Shujun LI Yongyi LUO Jun LI Yi 

机构地区：[1]School of Food Science and Pharmaceutical Engineering,Testing&Analysis Center,Nanjing Normal University,Nanjing 210023,China [2]Jiangsu Key Laboratory of Bioactive Natural Product Research and State Key Laboratory of Natural Medicines,China Pharmaceutical University,Nanjing 210009,China

出　　处：《Chinese Journal of Natural Medicines》2022年第11期846-853,共8页中国天然药物（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.81573550)。

摘　　要：Steroid saponins are secondary metabolites with multiple medicinal values that are found in large quantities in natural medicines,especially Vernonia amygdalina,a famous nature medicine for the treatment of tonsillitis,diabetes,pneumonia.The current study was designed to combine molecular networking(MN)with diagnostic ions for rapid identification ofΔ^(7,9(11))stigmastane-type saponins which were theα-glucosidase inhibitory active substances in V.amygdalina.First,theα-glucosidase inhibitory activities of fiveΔ^(7,9(11))stigmastane-type steroid saponins that were previously isolated were screened,which indicated that theΔ^(7,9(11))stigmastane-type steroid saponin was one of the active constituents responsible for ameliorating diabetes.Furthermore,a strategy was proposed to identify stigmastane-type steroid saponins and verify the plausibility of derived fragmentation pathways by applying MN,MolNetEnhancer and unsupervised substructure annotation(MS2LDA).Based on this strategy,other sevenΔ^(7,9(11))stigmastane-type steroid saponins were identified from this plant.Our research provide scientific evidence for the antidiabetic potential of the steroid saponin-rich extract of V.amygdalina leaf.

关 键 词：Δ^(7 9(11))Stigmastane-type steroid saponins α-Glucosidase inhibitory activity Fragmentation pathways Diagnostic ions Molecular networking 

分 类 号：R284[医药卫生—中药学] R965[医药卫生—中医学]