美洲大蠊提取物抑制肝癌HepG2细胞增殖的机制研究  

Mechanism Exploration of Periplaneta americana Extract on Inhibiting the Proliferation of HepG2 Cells

作　　者：吕洁 赵树立[2] 张雪峰 LYU Jie;ZHAO Shu-li;ZHANG Xue-feng(College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Clinical Research Center of Nanjing First Hospital,Nanjing 210012,China;Tripod Preclinical Research Laboratories Co.,Ltd.,Nanjing 210000,China)

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]南京市第一医院临床研究中心,江苏南京210012 [3]江苏鼎泰药物研究股份有限公司,江苏南京210000

出　　处：《现代中药研究与实践》2022年第5期37-42,共6页Research and Practice on Chinese Medicines

基　　金：南京市科技计划项目(201911001)。

摘　　要：目的探究美洲大蠊提取物对人肝癌细胞HepG2体内外的抑制作用及其可能的机制。方法采用噻唑蓝(MTT)法测定美洲大蠊提取物对HepG2细胞的增殖抑制率,流式细胞术检测美洲大蠊提取物对HepG2细胞的周期阻滞及凋亡诱导作用,通过Western Blotting检测细胞凋亡相关蛋白的表达;建立HepG2异种移植瘤模型,考察美洲大蠊提取物对肿瘤生长的影响。结果美洲大蠊提取物可抑制人肝癌细胞HepG2增殖,并可诱导人肝癌细胞HepG2细胞产生G2/M期阻滞及程序性细胞凋亡。蛋白印迹法显示美洲大蠊提取物通过调控凋亡相关蛋白发挥凋亡诱导作用。美洲大蠊提取物剂量依赖性抑制裸鼠HepG2异种移植瘤的生长。结论美洲大蠊提取物在体内外均能对HepG2细胞产生抑制作用,诱导细胞凋亡。Objective To explore the inhibitory effect of Periplaneta americana extract on human hepatoma cell line HepG2 in vitro and in vivo and its possible mechanism.Methods MTT assay was used to determine the proliferation inhibition rate of Periplaneta americana extract on HepG2 cells,flow cytometry was performed to detect the cycle arrest and apoptosis induction effect of Periplaneta americana extract on HepG2 cells.Western Blotting was used to detect the expression of apoptosis-related proteins.HepG2 xenograft tumor model was established to investigate the effect of Periplaneta americana extract on tumor growth.Results Periplaneta americana extract could inhibit the proliferation of HepG2 cells and induce G2/M block and programmed apoptosis of HepG2 cells.Western Blotting showed that Periplaneta americana extract exerted apoptosis induction by regulating apoptosis-related proteins.Periplaneta americana extract dose-dependently inhibited the growth of xenograft tumors of HepG2 in nude mice.Conclusion Periplaneta americana extract inhibits the proliferation of HepG2 cells in vitro and in vivo and induced cell apoptosis.

关 键 词：美洲大蠊提取物 肝癌 细胞凋亡 细胞周期 

分 类 号：R285.5[医药卫生—中药学]