妊娠期糖尿病对3月龄婴儿肠道菌群影响的前瞻性队列研究  

Effects of gestational diabetes mellitus on gut microbiota of 3-month-old infants:a prospective control study

作　　者：赵永丽 段一凡 杨振宇 张翠 牛蓓[1] 陈磊[1] 王敏伦 谷旭阳 梁广才 刘长青[1] Zhao Yongli;Duan Yifan;Yang Zhenyu;Zhang Cui;Niu Bei;Chen Lei;Wang Minlun;Gu Xuyang;Liang Guangcai;Liu Changqing(Hebei Province Center for Disease Control and Prevention,Shijiazhuang 050021,China;National Institute for Nutrition and Health,Chinese Center for Disease Control and Prevention,Beijing 100050,China;Wuqiang County Center for Disease Control and Prevention,Hengshui 053300,China;Health Supervision and Law Enforcement Bureau of Wuqiang County,Hengshui 053300,China;Institute of Microbiology,Chinese Academy of Sciences,Beijing 100080,China)

机构地区：[1]河北省疾病预防控制中心,石家庄050021 [2]中国疾病预防控制中心营养与健康所,北京100050 [3]武强县疾病预防控制中心,衡水053300 [4]武强县卫生健康综合监督执法局,衡水053300 [5]中国科学院微生物研究所,北京100080

出　　处：《卫生研究》2022年第6期934-939,共6页Journal of Hygiene Research

基　　金：河北省医学科学研究课题计划(No.20200699);国家部门预算财政项目《中国母婴营养与健康队列研究》。

摘　　要：目的比较妊娠期糖尿病(gestational diabetes mellitus,GDM)及正常孕妇的3月龄婴儿肠道菌群的差异,探讨妊娠糖尿病对后代肠道菌群的影响。方法数据来自中国母婴营养与健康队列,将2016年6月—2019年12月在河北省某县产检、分娩并随访至产后3个月的母亲及婴儿纳入研究,孕中期或孕晚期空腹血糖检测值≥5.1 mmol/L的孕妇及其婴儿纳入GDM组。采集3月龄婴儿粪便,采用16S rDNA高通量测序技术对所有粪便样本进行检测,了解两组肠道菌群分布特点。结果共纳入48对研究对象,GDM组16对,对照组32对。肠道菌群多样性比较:Alpha多样性指数分析,GDM组Observed Species指数(141.4±17.2)低于对照组(154.9±21.7),margalef指数(13.66±1.66)低于对照组(15.00±2.11),menhinick指数(0.83±0.10)低于对照组(0.91±0.13)(P<0.05)。菌落结构比较:在门水平上GDM组厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteria)和变形菌门(Proteobacteria)丰度分别为54.28(47.84)、23.58(23.48)、11.20(30.99)和0.12(0.07),对照组分别为42.05(36.23)、29.64(21.30)、9.31(15.82)和0.15(10.14),两组在门水平上均主要由这4个菌门组成,丰度最高的菌均为放线菌。在属水平上两组主要由5个菌属组成,分别是双歧杆菌属(Bifidobacteria)、乳杆菌属(lactobacillus)、肠杆菌属(Enterobacteria)、拟杆菌属(Bacteroidales)、梭菌属(Clostridiales)。GDM组的丹毒丝菌属(Erysipelotrichales)相对丰度0.01(0.01),显著低于对照组0.04(0.06)(P<0.05);GDM组的微球菌属(Micrococcales)相对丰度0.13(0.24),显著低于对照组0.29(0.78)(P<0.05)。PCoA分析:GDM组和对照组两组肠道菌群结构接近。LEfSe差异分析显示对照组丹毒梭菌属(Erysipelatoclostridium)显著升高(P<0.05)。结论妊娠期糖尿病会引起3月龄婴儿肠道菌群的多样性及丰富度降低,对主要菌门分布无显著性影响,对部分菌属分布有影响,提示GDM对稍大月龄婴儿肠道菌群构成仍OBJECTIVE To explore the effect of gestational diabetes mellitus on the gut microbiota of their offsprings,the gut microbiota of 3-month-old infants whose mothers diagnosed with and without gestational diabetes mellitus(GDM)was compared.METHODS Pregnant women and their 3-month-old infants who were examined,delivered and follow-up visited in a county in Hebei Province from June 2016 to December 2019 were included in our study.The pregnants and their infants with result of fasting venous blood glucose≥5.1 mmol/L in the second or third trimester were included in GDM group.Fecal samples of 3-month-old infants were collected and 16S rDNA high-throughput sequencing technology was used to explore the change of gut microbiota.RESULTS A total of 48 pairs of subjects were included,16 in GDM group and 32 in control group.Comparison of gut microbiota diversity:analysis of the Alpha diversity index showed that the Observed Species index,margalef index and menhinick index of GDM group were(141.4±17.2),(13.66±1.66)and(0.83±0.10),respectively,which were significantly lower than that of control group(154.9±21.7),(15.00±2.11)and(0.91±0.13),respectively.Sequence analysis:at phylum level,the abundance of the Firmicutes,Bacteroidetes,Actinobacteria and Proteobacteria were 54.28(47.84),23.58(23.48),11.20(30.99)and 0.12(0.07),respectively,while the control group were 42.05(36.23),29.64(21.30),9.31(15.82)and 0.15(10.14),respectively.The two groups were mainly composed of these 4 phyla and Actinobacteria was the most abundant.At genus level,there were mainly Bifidobacteria,lactobacillus,Enterobacteria,Bacteroidales and Clostridiales in both groups.The abundance of Erysipelotrichales in GDM group(0.01(0.01))was significantly lower than that in control group(0.04(0.06))(P<0.05).The abundance of Micrococcales in GDM group(0.13(0.24)was significantly lower than that in control group(0.29(0.78))(P<0.05).PCoA analysis showed that the gut microbiota structure of GDM group and control group was similar.LEfSe analysis showed Erysipelatocl

关 键 词：妊娠期糖尿病 肠道菌群 16S rDNA 高通量测序 

分 类 号：R587.1[医药卫生—内分泌] R181.3[医药卫生—内科学] R153[医药卫生—临床医学]