miR-182-5p attenuates Schistosoma japonicum-induced hepatic fibrosis by targeting tristetraprolin  

作　　者：Xuejun Zhao Zijie Xia Ziang Wang Mengsi Zhou Xuebing Qiu Cheng Wang Tian Xu Qian Fang Zhenping Ming Huifen Dong 

机构地区：[1]Hubei Province Key Laboratory of Allergy and Immunology,School of Basic Medical Sciences,Wuhan University,Wuhan 430071,China [2]Department of Medical Parasitology,School of Basic Medical Sciences,Wuhan University,Wuhan 430071,China [3]Kingstar Global,Wuhan 430070,China

出　　处：《Acta Biochimica et Biophysica Sinica》2022年第10期1421-1430,共10页生物化学与生物物理学报（英文版）

基　　金：supported by the grants from the National Natural Science Foundation of China(Nos.81971966 and 82172301).

摘　　要：Egg granuloma formation in the liver is the main pathological lesion caused by Schistosoma japonicum infection,which generally results in liver fibrosis and may lead to death in advanced patients.MicroRNAs(miRNAs)regulate the process of liver fibrosis,but the putative function of miRNAs in liver fibrosis induced by S.japonicum infection is largely unclear.Here,we detect a new miRNA,miR-182-5p,which shows significantly decreased expression in mouse livers after stimulation by soluble egg antigen(SEA)of S.japonicum or S.japonicum infection.Knockdown or overexpression of miR-182-5p in vitro causes the increased or decreased expression of tristetraprolin(TTP),an important immunosuppressive protein in the process of liver fibrosis.Furthermore,knockdown of miR-182-5p in vivo upregulates TTP expression and significantly alleviates S.japonicum-induced hepatic fibrosis.Our data demonstrate that downregulation of miR-182-5p increases the expression of TTP in mouse livers following schistosome infection,which leads to destabilization of inflammatory factor mRNAs and attenuates liver fibrosis.Our results uncover fine-tuning of liver inflammatory reactions related to liver fibrosis caused by S.japonicum infection and provide new insights into the regulation of schistosomiasis-induced hepatic fibrosis.

关 键 词：Schistosoma japonicum MICRORNA liver fibrosis miR-182-5p TRISTETRAPROLIN 

分 类 号：R532.21[医药卫生—内科学] R575.2[医药卫生—临床医学]