BSA技术与昆虫杀虫剂抗性基因定位  

BSA technology and mapping of insect insecticide resistance genes

作　　者：孟晨 仇鑫 陈斌[1] 张玉娟[1] MENG Chen;QIU Xin;CHEN Bin;ZHANG Yujuan(Chongqing Key Laboratory of Vector Insects,Institute of Entomology and Molecular Biology,Chongqing Normal University,Chongqing 401331,China)

机构地区：[1]重庆师范大学昆虫与分子生物学研究所媒介昆虫重庆市重点实验室,重庆401331

出　　处：《生命的化学》2022年第10期1828-1834,共7页Chemistry of Life

基　　金：国家自然科学基金项目(31872262,31672363,31871274)。

摘　　要：使用杀虫剂进行病虫害防控是有效防治虫媒疾病的重要方式之一,但大规模使用杀虫剂导致昆虫出现了不同程度的抗性。随着高通量测序技术的发展和分子标记的应用、大量物种全基因组测序的完成,利用双亲分离后代群体中具有极端表型的个体构建混池进行测序,通过比较不同混池之间的多态性并结合表型信息,从而定位目的基因的混合群体分离分析(bulked segregant analysis,BSA)技术,因其简单、高效的特点得到了快速广泛的应用。采用BSA技术揭示杀虫剂抗性产生的分子机制,对抗药性产生风险的有效评估以及新型杀虫剂的改良开发具有重要意义。本文就近年来BSA技术的发展和昆虫杀虫剂抗性分子机理的相关研究进行综述,并对该技术在昆虫杀虫剂抗性研究中的意义和应用前景进行了总结与展望。The use of pesticides for pest control is one of the important ways to effectively control insectborne diseases, but the large-scale use of pesticides has led to the emergence of different degrees of resistance in insects. With the development of high-throughput sequencing technology, the application of molecular markers, and the completion of whole-genome sequencing of a large number of species, individuals with extreme phenotypes in the parental segregation progeny population are used to construct mixed pools for sequencing. By comparing the polymorphisms between different mixed pools, bulked segregant analysis(BSA) technology, which combines phenotypic information to locate target genes, has been rapidly and widely used because of its simplicity and high efficiency. The use of BSA technology to reveal the molecular mechanism of pesticide resistance, effective assessment of the risk of pesticide resistance, the improvement and development of new pesticides are of great significance. In this paper, the development of BSA technology and related research on the molecular mechanism of insect insecticide resistance in recent years are reviewed,and the significance and application prospect of this technology in insect insecticide resistance research are summarized and prospected.

关 键 词：混合群体分离分析技术 单核苷酸多态性标记 杀虫剂抗性 基因定位 

分 类 号：S433[农业科学—农业昆虫与害虫防治]