油用型向日葵矮大头杂交种染色体核型分析  

Karyotype Analysis of Oil Sunflower Hybrid Aidatou

作　　者：马军[1] 宋承泽 丁海燕 汪洪敏 任国领 段立秋 赵天彪 李志勇[5] MA Jun;SONG Chengze;DING Haiyan;WANG Hongmin;REN Guoling;DUAN Liqiu;ZHAO Tianbiao;LI Zhiyong(Institute of Industrial Crops,Heilongjiang Academy of Agricultural Sciences,Harbin 150086;College of Horti-culture,China Agricultural University,Beijing 100193;Heilongjiang Provincial Key Laboratory of Oilfield Applied Chemistry and Technology/School of Biological Engineering,Daqing Normal University,Daqing 163712;Life Sci-ence and Technology Academy,Harbin Normal University,Harbin 150080;Institute of Plant Protection,Heilongji-ang Academy of Agricultural Sciences,Harbin 150086,China)

机构地区：[1]黑龙江省农业科学院经济作物研究所,哈尔滨150086 [2]中国农业大学园艺学院,北京100193 [3]黑龙江省油田应用化学与技术重点实验室/大庆师范学院生物工程学院,黑龙江大庆163712 [4]哈尔滨师范大学生命科学与技术学院,哈尔滨150080 [5]黑龙江省农业科学院植物保护研究所,哈尔滨150086

出　　处：《东北农业科学》2022年第5期47-49,共3页Journal of Northeast Agricultural Sciences

基　　金：国家重点研发计划(2017YFD0200607);国家现代农业技术体系建设项目(CARS-14);大庆师范学院科学研究基金(19ZR15)。

摘　　要：为了探索油用型向日葵根尖细胞染色体制片技术及核型分析的方法,本研究以油用矮大头杂交种种子为研究材料,采用去壁低渗火焰干燥法和常规染色制片法进行比较,试验表明常规染色制片效果较好,可以用于核型分析,矮大头向日葵F1种子的染色体核型为2n=34=24m(Sat)+2St+8Sm(Sat)。In order to explore the techniques of chromosome production and karyotype analysis of sunflower root tip cells,the Aidatou seed of sunflower was used as the research material.The experiments were performed using the dehulled hypotonic flame drying method and the conventional dyeing method.The results showed that the conventional dyeing method was better and could be used for karyotype analysis.Chromosome analysis of sunflower chromosomes was performed by selecting clear chromosome images.The karyotype of Aidatou F1 seed was 2n=34=24m(Sat)+2St+8Sm(Sat).

关 键 词：向日葵 染色体 核型分析 

分 类 号：S565.5[农业科学—作物学]