斯巴坦蓝莓叶片离体再生研究  

Study on in vitro Regeneration of Blueberry Leaves from"Spartan"

作　　者：叶霞 孙世海[1] 郭茹 华仁锐 张桂霞[1] YE Xia;SUN Shihai;GUO Ru;HUA Renrui;ZHANG Guixia(Tianjin Agricultural College,Tianjin 300384,China)

机构地区：[1]天津农学院,天津300384

出　　处：《东北农业科学》2022年第5期50-52,共3页Journal of Northeast Agricultural Sciences

基　　金：天津农学院研究生培养质量提升项目(2017YPY006);天津市科技计划项目(17ZXBFNC00210)。

摘　　要：以斯巴坦蓝莓试管苗叶片为试材,WPM为基本培养基,研究细胞分裂素ZT、TDZ与生长素IBA、NAA的激素组合及暗培养时间对其叶片离体再生体系建立的影响。试验结果表明,当TDZ为0.02 mg/L、IBA为0.3 mg/L时,诱导产生平均芽数为4.61,在所有处理中最高,且分化芽时间快,芽丛密度大;暗培养20 d的愈伤组织诱导率最高为100%,且愈伤组织体积最大。综合分析得出,较适宜斯巴坦蓝莓叶片分化的培养基为WPM+TDZ 0.02 mg/L+IBA 0.3 mg/L,适宜斯巴坦蓝莓叶片愈伤组织诱导的暗培养时间为20 d。The effects of the hormone combination of cytokinin ZT,TDZ,auxin IBA,NAA and dark culture time on the establishment of regeneration system of in vitro leaves of"Spartan"blueberry were studied.The results showed that when TDZ was 0.02 mg/L and IBA was 0.3 mg/L,the average number of buds induced was 4.61,which was the highest in all treatments,and the time of bud differentiation was fast,and the density of bud cluster was large;the highest callus induction rate was 100%in dark culture for 20 d,and the largest callus.Comprehensive analysis showed that the suitable medium for leaf differentiation of"Spartan"blueberry was WPM+TDZ 0.02 mg/L+IBA 0.3 mg/L,and the suitable dark culture time for leaf callus induction of"Spartan"blueberry was 20 d.

关 键 词：蓝莓 叶片 愈伤组织 生长调节剂 暗培养 

分 类 号：S663.9[农业科学—果树学]