6-姜酚抗大鼠运动病作用的内耳机制研究  

Study on inner ear mechanism of 6-gingerol for resisting motion sickness in rats

作　　者：马永勤 刘琦 徐丽华[1] 王国华[1] 周鑫 姜正林[1] Ma Yongqin;Liu Qi;Xu Lihua;Wang Guohua;Zhou Xin;Jiang Zhenglin(Institute of Special Environmental Medicine,Nantong University,Nantong 226019,China)

机构地区：[1]南通大学特种医学研究院,南通226019

出　　处：《中华航海医学与高气压医学杂志》2022年第5期587-591,共5页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：国家自然科学基金青年项目(81801867);国家自然科学基金面上项目(81372131,82171869)。

摘　　要：目的探讨生姜有效成分6-姜酚抗运动病作用的内耳相关机制。方法SD大鼠30只,按照实验设计分为对照组、旋转组、6-姜酚(50 mg/kg)组、6-姜酚+钌红(5 mg/kg)组、单独钌红(5 mg/kg)组,每组6只。按照实验设计旋转刺激SD大鼠,诱导其产生对0.15%糖精溶液的条件性厌饮行为,模拟运动病。利用qRT-PCR、Western blotting、ELISA等生物技术检测大鼠内耳水通道蛋白2(AQP2)、血浆精氨酸加压素(AVP)的V2受体(V2R)和瞬时感受器电位离子通道4(TRPV4)mRNA和蛋白的表达水平,并观察TRPV4拮抗剂钌红对6-姜酚抗运动病效果的影响。结果6-姜酚抑制旋转刺激后,大鼠内耳AQP2 mRNA和蛋白的表达水平明显升高(P<0.05),TRPV4 mRNA和蛋白表达水平明显降低(P<0.05或P<0.01),并降低V2R mRNA和蛋白的表达水平(P<0.05)。单独使用钌红(5 mg/kg)诱导,大鼠对糖精溶液的饮用量明显减少(P<0.01);在给予6-姜酚的同时使用钌红(5 mg/kg),则消除了6-姜酚预处理对旋转刺激后大鼠糖精溶液饮用量减少的抑制作用(P<0.01)。结论6-姜酚的抗运动病机制可能与其影响内耳V2R、TRPV4的转录和表达,进而影响AQP2的表达有关。Objective To explore inner ear mechanism of 6-gingerol,an active ingredient of ginger,for resisting motion sickness in rats.Methods By stimulating SD rats with rotation,so as to induce conditioned anorexia to 0.15%saccharin solution,thus simulating motion sickness.Using qRT-PCR,western blotting,ELISA,and other related biological techniques to detect aquaporin 2(AQP2),plasma arginine vasopressin(AVP)V2 receptor(V2R),transient receptor potential ion channel 4(TRPV4)mRNA,and protein expression levels of the rats’inner ears,and observe the impact of TRPV4 antagonist ruthenium red on the effect of 6-gingerol countering motion sickness.Results After 6-gingerol inhibited the rotational stimulation,the expression levels of AQP2 mRNA and protein in the inner ear of rats were significantly increased(P<0.05),the TRPV4 mRNA and protein expression levels were significantly decreased(P<0.05),and the expression levels of V2R mRNA and protein were decreased(P<0.05).After using ruthenium red(5 mg/kg)alone,the reduction of drinking saccharin in rats was significant(P<0.01).The 6-gingerol combing with ruthenium red(5 mg/kg)could eliminate the inhibitory effect of 6-gingerol pretreatment on the reduction of saccharin drinking in rats after rotational stimulation(P<0.01).Conclusion The mechanism of 6-gingerol for resisting motion sickness may be related to its impact on the transcriptions and expressions of V2R and TRPV4 in inner ear,and then on the AQP2 expression.

关 键 词：运动病 6-姜酚 内耳 水通道蛋白2 V2受体 瞬时感受器电位离子通道4 

分 类 号：R285.5[医药卫生—中药学]