旋转刺激对大鼠内耳α-ENaC蛋白表达的影响  

Effect of rotational stimulation on the expression of α-ENaC in the inner ear of rats

作　　者：任畅 宋佳云 周鑫 徐丽华[1] 姜正林[1] Ren Chang;Song Jiayun;Zhou Xin;Xu Lihua;Jiang Zhenglin(Institute of Special Environmental Medicine,Nantong University,Nantong 226019,China)

机构地区：[1]南通大学特种医学研究院,南通226019

出　　处：《中华航海医学与高气压医学杂志》2022年第5期592-596,共5页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：国家自然科学基金青年项目(81801867);国家自然科学基金面上项目(81372131,82171869)。

摘　　要：目的探讨旋转刺激对上皮钠通道α亚基(α-ENaC)蛋白在大鼠内耳不同部位表达分布的影响。方法以12只SD大鼠为实验对象,按照实验设计分为对照组和实验组,每组6只。对照组不作任何处理;实验组旋转刺激后采用免疫组化染色观察α-ENaC蛋白在其内耳不同部位的表达变化。结果旋转刺激后,实验组α-ENaC蛋白在大鼠耳蜗血管纹、螺旋神经节神经元胞体、周围卫星细胞的表达较对照组有所增加,在前庭部位的椭圆囊斑上皮和基质细胞及前庭神经节的神经元胞体中的表达也有所增加,在半规管壶腹嵴上皮和基质细胞中的分布有所减少,而在耳蜗螺旋韧带和赖斯纳氏膜、前庭部位的球囊斑上皮和基质细胞以及半规管上皮细胞中的分布基本不变。结论α-ENaC蛋白在大鼠内耳中广泛表达,且旋转刺激后在不同部位的表达变化存在差异;ENaC可能通过对内耳内淋巴平衡和前庭觉的调控参与运动病的发生。Objective To investigate the effect of rotational stimulation on the expression and distribution of α-ENaC in different areas of the inner ears of rats.Methods With immunohistochemistry staining,the expressions of α-ENaC in different areas of the inner ears of the Sprague-Dawley rats after rotational stimulation were observed.Results After rotational stimulation,the expressions of α-ENaC in the stria vascularis,the spiral ganglion neurons,peripheral satellite cells,the epithelial and stromal cells of the macula utriculi,the vestibular ganglion neurons of rats were increased,while the distributions of α-ENaC in epithelial and stromal cells of the crista ampullaris were decreased.Moreover,in the spiral ligament,Reissner’s membrane,the epithelial and stromal cells of the macula sacculi,and the epithelial cells of the semicircular canal,the distributions of α-ENaC of the observation group were similar to the control group.Conclusion The α-ENaC were widely expressed in the inner ears,and the changes of its expression varied among different areas in the inner ears of rats after rotation.Thus,ENaC may be involved in the development of motion sickness through regulating the endolymph balance and vestibular sensation of the inner ears.

关 键 词：上皮钠通道 内耳 旋转刺激 前庭 内淋巴液 

分 类 号：R594.6[医药卫生—内科学]