Comparative phosphoproteomics reveal new candidates in the regulation of spermatogenesis of Drosophila melanogaster  

作　　者：Bin Mao Wei Zhang Ya Zheng Dong Li Meng-Yan Chen Yu-Feng Wang 

机构地区：[1]School of Life Sciences,Hubei Key Laboratory of Genetic Regulation and Integrative Biology,Central China Normal University,Wuhan,China

出　　处：《Insect Science》2022年第6期1703-1720,共18页昆虫科学（英文版）

基　　金：supported by the National Natural Science Foundation of China(no.31672352,31872288).

摘　　要：The most common phenotype induced by the endosymbiont Wolbachia in in-sects is cytoplasmic incompatibility,where none or fewer progenies can be produced when Wolbachia-infected males mate with uninfected females.This suggests that some modi-fications are induced in host sperms during spermatogenesis by Wolbachia.To identify the proteins whose phosphorylation states play essential roles in male reproduction in Drosophila melanogaster,we applied isobaric tags for relative and absolute quantitation(iTRAQ)-based proteomic strategy combined with titanium dioxide(TiO2)enrichment to compare the phosphoproteome of Wolbachia-infected with that of uninfected male re-productive systems in D.melanogaster.We identified 182 phosphopeptides,defining 140 phosphoproteins,that have at least a 1.2 fold change in abundance with a P-value of<0.05.Most of the differentially abundant phosphoproteins(DAPPs)were associated with micro-tubule cytoskeleton organization and spermatid differentiation.The DAPPs included pro-teins already known to be associated with spermatogenesis,as well as many not previously studied during this process.Six genes coding for DAPPs were knocked down,respectively,in Wolbachia-free fly testes.Among them,Slmap knockdown caused the most severe dam-age in spermatogenesis,with no mature sperm observed in seminal vesicles.Immunoflu-orescence staining showed that the formation of individualization complex composed of actin cones was completely disrupted.These results suggest that Wolbachia may induce wide changes in the abundance of phosphorylated proteins which are closely related to male reproduction.By identifying phospho-modulated proteins we also provide a signifi-cant candidate set for future studies on their roles in spermatogenesis.

关 键 词：Drosophila melanogaster individualization complex PHOSPHOPROTEOME Slmap SPERMATOGENESIS 

分 类 号：Q96[生物学—昆虫学]