Population density modulates insect progenitive plasticity through the regulation of dopamine biosynthesis  

作　　者：Kai Liu Longyu Yuan Lei Yue Weiwen Chen Kui Kang Jun Lv Wenqing Zhang Rui Pang 

机构地区：[1]State Key Laboratory of Biocontrol,School of Life Sciences,Sun Yat-sen University,Guangzhou,China [2]Guangdong Provincial Key Laboratory of Microbial Safety and Health,State Key Laboratory of Applied Microbiology Southern China,Institute of Microbiology,Guangdong Academy of Sciences,Guangzhou,China [3]Innovative Institute for Plant Health,College of Agriculture and Biology,Zhongkai Universityof Agriculture and Engineering,Guangzhou,China

出　　处：《Insect Science》2022年第6期1773-1789,共17页昆虫科学（英文版）

基　　金：supported by the National Natural Science Foundation of China(31672021 and 31730073);Applied Basic Research Programs of Science and Technology Commission Foundation of Guangdong Province(2019A1515110867 and 2020A1515110063);Research Project of Innovative Institute for Plant Health(KA21031H104);Guangdong University Key Laboratory for Sustainable Control of Fruit and Vegetable Diseasesand Pests(2020KSYS005).

摘　　要：Insect fecundity is a quantitative phenotype strongly affected by genotypes and the environment.However,interactions between genotypes and environmental factors in modulating insect fecundity remain largely unknown.This study investigated the im-pact of population density on the fecundity of Nilaparvata lugens(brown planthopper;BPH)carrying homozygous high-(HFG)or low-(LFG)fecundity homozygous geno-types.Under low population densities,the fecundity and population growth rate of both genotypes showed similar increasing trends across generations,while the trends between HFG and LFG under high population densities were opposite.Through a combination of temporal analysis and weighted gene co-expression network analyses on RNA-seq data of HFG and LFG under low and high population densities in the lst,3rd,and 5th gener-ations,we identified 2 gene modules that were associated with these density-dependent progenitive phenotypes.Four pathways related to the neural system were simultaneously enriched by the 2 gene modules.Furthermore,Nlpale,which encodes a tyrosine hydroxy-lase,was identified as a key gene.The RNA interference of this gene and manipulation of its downstream product dopamine significantly affected the basic and density-dependent progenitive phenotypes of BPH.These findings indicated that dopamine biosynthesis is the key regulatory factor that determines fecundity in response to density changes in dif-ferent BPH genotypes.Thus,this study provides insights into the interaction of a typical environmental factor and insect genotype during the process of population regulation.

关 键 词：dopamine biosynthesis FECUNDITY GENOTYPE Nilaparvata lugens popula-tiondensity 

分 类 号：Q96[生物学—昆虫学]