鸭疫里默氏杆菌流行菌株的分离鉴定及生物学特性  

Isolation, identification, and biological characterization of Riemerella anatipestifer epidemic strains

作　　者：冯雅婷 朱敏 刘丹 沈如玉 牛朋飞 郭容 于圣青[1,3] FENG Yating;ZHU Min;LIU Dan;SHEN Ruyu;NIU Pengfei;GUO Rong;YU Shengqing(Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,China;College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,Anhui,China;Yangzhou Youjiachuang Biotechnology Limited Company,Yangzhou 225261,Jiangsu,China)

机构地区：[1]中国农业科学院上海兽医研究所,上海200241 [2]安徽农业大学动物科技学院,安徽合肥230036 [3]扬州优佳创生物科技有限公司,江苏扬州225261

出　　处：《微生物学通报》2022年第11期4778-4785,共8页Microbiology China

基　　金：上海市科技创新行动计划(19391902800)。

摘　　要：【背景】鸭疫里默氏杆菌(Riemerella anatipestifer,RA)是引起雏鸭、鹅、火鸡等多种家禽、野禽发生传染性浆膜炎的病原,在全世界范围内广泛存在,危害养禽业发展,造成严重的经济损失。【目的】了解国内RA的流行现状,探究该菌的生物学特性,更好地指导防控鸭疫里默氏杆菌病。【方法】对2020-2021年从山东省、河北省、广东省、山西省等地分离的RA疑似菌株进行PCR、生化和血清型鉴定,并根据药物敏感性结果分析耐药现象,根据半数致死量(median lethal dose,LD_(50))测定结果分析致病力差异。【结果】共鉴定78株RA分离株,其中,血清1型4株、2型21株、10型11株、6型3株、7型17株,1株有交叉凝集现象,21株血清型未定型;药敏结果显示78株分离株对多粘菌素B、磷霉素、克林霉素等的耐药性最强,对头孢拉定、多西环素、呋喃唑酮、氟苯尼考等抗生素最为敏感,并且78株分离株均存在多重耐药性,其中77株耐药5 重及以上;动物试验结果显示,RA的分离株致病力普遍较强,而且不同地区分离株、同地区不同分离株之间均存在差异,数株RA分离株的LD_(50)在10^(4)-10^(7) CFU不等。【结论】RA在国内流行表现了强致病力及严重耐药情况,本研究结果为更好地预防控制、临床用药及后续致病机制研究提供了参考依据。[Background] Riemerella anatipestifer(RA) is the pathogen that causes infectious serositis in a variety of domesticated and wild birds such as ducklings, geese, and turkeys. With wide distribution all over the world, it endangers the development of poultry industry and causes serious economic losses.[Objective] To learn the epidemic status and biological characteristics of RA in China and guide the prevention and control of RA-caused disease. [Methods] PCR, biochemical tests, and serotyping were carried out for the suspected RA strains isolated from Shandong province, Hebei province, Guangdong province, and Shanxi province from 2020 to 2021. The drug resistance of the strains was evaluated by drug sensitivity test, and the pathogenicity was analyzed based on median lethal dose. [Results] A total of 78 RA strains were identified, including 4 strains of serotype 1, 21 strains of serotype 2, 11 strains of serotype 10, 3 strains of serotype 6, and 17 strains of serotype 7, 1 strain with cross agglutination, and 21 strains not serotyped. The 78 strains had the strong drug resistance to polymyxin B, fosfomycin, and clindamycin and were sensitive to cefradine, doxycycline, furazolidone, and florfenicol. In addition, all the 78 strains had multiple drug resistance, among which 77 strains had resistance to more than 5 antibiotics. The animal test results showed that the RA strains generally had strong pathogenicity,which varied among different strains of the same region and among strains of different regions. The LD_(50) of 14 RA strains ranged from 10^(4) to 10^(7) CFU. [Conclusion] RA has demonstrated strong pathogenicity and drug resistance in China. The results of this study provide a basis for the prevention and control, clinical medication, and subsequent research on the pathogenic mechanism of RA.

关 键 词：鸭疫里默氏杆菌 分离 鉴定 耐药性 致病性 

分 类 号：S852.61[农业科学—基础兽医学]