健康老年人肠道菌群宏基因组与16S rDNA测序的比较研究  

Comparative analysis of metagenomic and 16S rDNA sequencing in gut microbiota of healthy elderly

作　　者：庄思琪 毛怡心 邓富昌 雒月云 石婉荧 李霞[2] 曹亚强 徐继承 唐宋 Zhuang Siqi;Mao Yixin;Deng Fuchang;Luo Yueyun;Shi Wanying;Li Xia;Cao Yaqiang;Xu Jicheng;Tang Song(Department of Epidemiology and Health Statistics,School of Public Health,Xuzhou Medical University,Xuzhou 221004,China;China CDC Key Laboratory of Environment and Population Health,National Institute of Environmental Health,Chinese Center for Disease Control and Prevention,Beijing 100021,China;Center for Global Health,School of Public Health,Nanjing Medical University,Nanjing 211166,China)

机构地区：[1]徐州医科大学公共卫生学院流行病与卫生统计学教研室,徐州221004 [2]中国疾病预防控制中心环境与健康相关产品安全所环境与人群健康重点实验室,北京100021 [3]南京医科大学公共卫生学院全球健康中心,南京211166

出　　处：《中华预防医学杂志》2022年第11期1618-1624,共7页Chinese Journal of Preventive Medicine

基　　金：大气重污染成因与治理攻关项目(DQGG0401);中国疾病预防控制中心环境所青年科学基金项目(2020YSRF_03)。

摘　　要：目的基于健康老年人群的肠道菌群组成特征,探索宏基因组测序与16S rDNA测序在后续分析上的差异。方法采用定群研究设计,对山东济南76名健康老年人进行5次重复测量,采集粪便样本并提取基因组DNA,分别进行宏基因组与16S rDNA测序,比较两种测序肠道菌群的组成与多样性;采用Pearson相关分析物种丰度与α多样性的相关性,普氏分析判断β多样性的相关性。结果76名老年人年龄(65.07±2.75)岁,体重指数为(25.03±2.40)kg/m^(2);男、女各38名;5次重复测量共得到345人次的粪便样品。相较于16Sr DNA测序,宏基因组测序在各个水平的注释物种数更多,且水平越低,两种测序物种数量相差越大;两种测序的物种丰度存在差异,但在门(r=0.88,P<0.001)、属(r=0.77,P<0.001)水平上物种丰度呈高度相关,其中拟杆菌门、厚壁菌门等是共同的优势物种;老年人肠道菌群多样性分析显示,两种测序的α多样性指数存在显著正相关(r=0.70,P<0.001),两组β多样性也呈显著相关(M^(2)=0.84,P<0.05);结论宏基因组测序注释效率远高于16S rDNA测序;两种测序方法在门水平物种丰度、物种α多样性上具有较高的一致性。Objective To explore the differences in subsequent analysis between metagenomic and 16Sr DNA sequencing in compositionally characterizing gut microbiota of healthy elderly.Methods By using a panel study design,five monthly repeated measurements were performed among 76 healthy older people in Jinan City,Shandong Province.Their fecal samples were collected,and genomic DNA was extracted and analyzed through metagenomic and 16Sr DNA sequencing to compare the composition and diversity of gut microbiota.The correlation between species abundance andαdiversity was analyzed by Pearson correlation analysis,and the correlation between species abundance andβdiversity was determined by Procrustes analysis.Results The age of 76 participants was(65.07±2.75),and the body mass index was(25.03±2.40)kg/m^(2).There were 38 males and 38 females.A total of 345 fecal samples were obtained from five monthly repeated measurements.Compared with 16S rDNA sequencing,metagenomic sequencing showed more annotated species at each level.The difference in the number of two sequencing species increased with the decrease of the level.Although there were significant differences in species richness between the two sequencing methods.Their species richness was highly correlated at both phylum(r=0.88,P<0.001)and genus(r=0.77,P<0.001)levels.Bacteroidetes and Firmicutes were the common dominant species.Gut microbiota diversity analysis further showed that there was a significantly positive correlation betweenαdiversity(r=0.70,P<0.001)andβdiversities(M^(2)=0.84,P<0.05)in the two groups.Conclusion The annotation efficiency of metagenomic sequencing is much higher than that of 16S rDNA sequencing.The two sequencing methods are consistent in phylum abundance as well asαdiversity.

关 键 词：老年人 肠道微生物 多样性 高通量测序 

分 类 号：R37[医药卫生—病原生物学]