β淀粉样前体蛋白裂解酶1在大鼠颈上神经节组织中的表达  

Expression of β-site amyloid precursor protein cleaving enzyme 1 in rat superior cervical ganglion tissues

作　　者：范娅楠 李超红[1] 贾祥磊[1] 金家豪 刘玉珍[1] FAN Ya-nan;LI Chao-hong;JIA Xiang-lei;JIN Jia-hao;LIU Yu-zhen(He'nan Key Laboratory of Neural Regeneration,the First Affiliated Hospital of Xinxiang Medical University,He'nan Weihui 453100,China;Department of Clinical Laboratory,Zhengzhou Sixth People's Hospital,Zhengzhou 450000,China)

机构地区：[1]新乡医学院第一附属医院河南省神经修复重点实验室,河南卫辉453100 [2]郑州市第六人民医院检验科,郑州450000

出　　处：《解剖学报》2022年第6期698-704,共7页Acta Anatomica Sinica

基　　金：国家自然科学基金(81973988,81470271);新乡医学院研究生科研创新支持计划项目(YJSCX201847Y);新乡医学院第一附属医院青年基金(QN-2017-A008)。

摘　　要：目的 观察β淀粉样前体蛋白裂解酶1(BACE1)在大鼠颈上神经节的表达和定位,及慢性间歇性低氧(CIH)对BACE1表达水平的影响。方法 采用RT-PCR、Western blotting和免疫组织化学法检测颈上神经节BACE1的表达及分布。16只雄性SD大鼠随机分为对照(control)组和CIH组,每组8只,模型制作2周,RT-PCR检测CIH对BACE1、过氧化物酶体增殖物激活受体γ辅激活因子1α(PGC-1α)mRNA水平的影响。结果 BACE1表达于大鼠颈上神经节,主要分布于卫星胶质细胞和神经纤维,而在血管、神经元和小强荧光(SIF)细胞不表达。CIH下调BACE1 mRNA水平,上调PGC-1αmRNA水平(P<0.01)。结论 BACE1定位于大鼠颈上神经节卫星胶质细胞和神经纤维;BACE1水平降低可能参与调节CIH诱导的颈上神经节突触的可塑性。Objective To observe the expression and localization of β-site amyloid precursor protein cleaving enzyme 1(BACE1) in rat superior cervical ganglion and the effect of chronic intermittent hypoxia(CIH) on BACE1 level. Methods The expression and distribution of BACE1 in superior cervical ganglion were detected by RT-PCR, Western blotting and immunohistochemistry. Totally 16 male SD rats were randomly divided into control group and CIH group, 8 rats in each group. After 2 weeks of modeling, the effect of CIH on BACE1 and peroxisome proliferators activated receptor gamma co-activator 1 alpha(PGC-1α) mRNA level was detected by RT-PCR. Results BACE1 was expressed in rat superior cervical ganglion, and mainly distributed in satellite glial cells and nerve fibers, but not in blood vessels, neurons and small intesely fluorecent(SIF) cells. CIH down-regulated BACE1 mRNA level, but up-regulated PGC-1α mRNA level(P<0.01). Conclusion BACE1 is located in satellite glial cells and nerve fibers in the superior cervical ganglion of rats. The decreased level of BACE1 may be involved in the regulation of CIH-induced synaptic plasticity of superior cervical ganglion.

关 键 词：β淀粉样前体蛋白裂解酶1 颈上神经节 慢性间歇性低氧 免疫印迹法 大鼠 

分 类 号：R34[医药卫生—基础医学]