电针对局灶性脑缺血/再灌注大鼠大脑皮质胶质瘢痕形成的影响  

Effect of electroacupuncture on the formation of glial scars in cerebral cortex of rats with focal cerebral ischemia/reperfusion

作　　者：胥虹贝 罗勇[2] XU Hong-bei;LUO Yon(Department of Neurology,Affiliated Hospital of Guizhou Medical University,Guiyang 550004,China;Department of Neurology,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]贵州医科大学附属医院神经内科,贵阳550004 [2]重庆医科大学附属第一医院神经内科,重庆400016

出　　处：《解剖学报》2022年第6期705-710,共6页Acta Anatomica Sinica

基　　金：国家自然科学青年基金(82001270);贵州省科技计划基础研究项目(黔科合基础-ZK[2021]一般413);国家自然科学地区基金(82160242);贵州医科大学附属医院博士科研启动基金(gyfybsky-2021-23)。

摘　　要：目的 观察电针对局灶性脑缺血/再灌注(I/R)大鼠大脑皮质胶质瘢痕形成的影响。方法 采用线栓法构建局灶性脑缺血/再灌注模型,选取“百会”穴(GV 20)及左侧“四关”穴(合谷LI 4/太冲LR 3)为电针穴位。78只雄性SD大鼠随机分为假手术组(sham)、局灶性脑缺血/再灌注(I/R)组、局灶性脑缺血/再灌注+电针(I/R+EA)组。再灌注后7 d,采用改良神经功能缺损评分(mNSS)评估神经功能缺损情况,HE染色观察缺血侧大脑皮质损伤情况,免疫荧光标记缺血侧大脑皮质胶质纤维酸性蛋白(GFAP)^(+)/神经蛋白聚糖(neurocan)^(+)、GFAP^(+)/磷酸肌酸蛋白聚糖(phosphacan)^(+)细胞,分析各组GFAP、neurocan及phosphacan平均免疫荧光强度。采用Real-time PCR分析各组缺血侧大脑皮质GFAP、neurocan及phosphacan mRNA含量。结果 与I/R组相比,I/R+EA组大鼠mNSS评分明显降低(P<0.01),脑组织损伤程度明显减轻。Sham组GFAP^(+)/neurocan^(+)、GFAP^(+)/phosphacan^(+)细胞非常少。I/R组星形胶质细胞胞体肥大,细胞突起增多、增粗,GFAP^(+)/neurocan^(+)和GFAP^(+)/phosphacan^(+)细胞明显多,而I/R+EA组GFAP^(+)/neurocan^(+)和GFAP^(+)/phosphacan^(+)细胞明显较I/R组减少。此外,I/R+EA组GFAP、neurocan及phosphacan平均免疫荧光强度,以及上述各指标的mRNA含量均明显低于I/R组(P<0.05)。结论 电针可促进局灶性脑I/R大鼠神经功能恢复,减轻脑组织损伤,这可能与电针抑制大脑皮质胶质瘢痕形成有关。Objective To observe the effect of electroacupuncture on the formation of glial scars in the cerebral cortex of rats with focal cerebral ischemia/reperfusion(I/R). Methods The focal cerebral ischemia/reperfusion model was established by thread method, and “Baihui”(GV 20) and left “Siguan”(Hegu LI 4/Taichong LR 3) were selected as acupoints points. Seventy-eight male SD rats were randomly divided into sham group(sham), focal cerebral ischemia/reperfusion(I/R), focal cerebral ischemia/reperfusion+ electroacupuncture group(I/R+EA). Seven days after reperfusion, modified neurological severity score(mNSS) was used to evaluate neurological deficit, HE staining was used to observe the degree of ischemic cerebral cortex. Immunofluorescencte was used to observe glial fibrillary acidic protein(GFAP)/neurocan, GFAP/phosphacan positive cells in the ischemic cerebral cortex. The average immunofluorescent intensity of GFAP, neurocan and phosphacan, and the levels of GFAP, neurocan and phosphacan mRNA in the ischemic cerebral cortex were analyzed by Real-time PCR, respectively. Results Compared with the I/R group, mNSS score in I/R+EA group decreased significantly(P<0.01), and the degree of brain tissue damage reduced obviously. There were very few GFAP/neurocancells and GFAP/phosphacancells in the sham group. The astrocytes in I/R group were hypertrophy, increasing and thickened protrusions, more GFAP/neurocancells and GFAP/phosphacancells were dectected as well. While the count of GFAP/neurocancells and GFAP/phosphacancells in I/R+EA group were significantly lower than those in I/R group. In addition, the average immunofluorescence intensity of GFAP, neurocan and phosphacan in I/R+EA group, and mRNA content of above mentioned indicators were significantly lower than those in I/R group(P<0.05). Conclusion Electroacupuncture promotes the recovery of neurological function in rats with focal cerebral I/R and reduce brain tissue damage, which may be related to the inhibition of glial scar formation in cerebral cortex.

关 键 词：局灶性脑缺血/再灌注 胶质瘢痕 电针 实时定量聚合酶链反应 大鼠 

分 类 号：R245.97[医药卫生—针灸推拿学]