基于网络药理学探究人参-黄芪治疗缺血性心肌病的作用机制  

Exploring the action mechanism of Renshen-Huangqi medicine pair in the treatment of ischemic cardiomyopathy based on network pharmacology

作　　者：化振鑫 蒋海洋 吴文胜[2] HUA Zhenxin

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《中医临床研究》2022年第32期61-66,共6页Clinical Journal Of Chinese Medicine

摘　　要：目的:探讨人参-黄芪治疗缺血型心肌病的作用机制。方法:使用中药系统药理学数据库与分析平台(TCMSP)检索人参、黄芪的有效成分和相关靶点,利用Cytoscape软件构建药物-活性成分-关键靶点”网络,从STRING平台生成蛋白质-蛋白质相互作用网络,在Metascape数据库进行基因本体论(GO)富集分析及京都基因与基因组百科全书(KEGG)通路富集分析。结果:化学成分-靶点网络包含42个化合物和718个相应靶点,100个药物交集靶点,药物活性成分主要为槲皮素、山柰酚、7-O-甲基-异微凸剑叶莎醇、芒柄花素、异鼠李素等。蛋白质-蛋白质相互作用网络关键靶点为丝氨酸/苏氨酸蛋白激酶1(Akt Serine/Threonine Kinase 1,AKT1)、白细胞介素(Interleukin,IL)-6、肿瘤坏死因子(Tumor Necrosis Factor,TNF)、半胱氨酸天冬氨酸蛋白酶3(Caspase 3,CASP3)、IL-1β、肿瘤蛋白p53(Tumor Protein p53,TP53)、血管内皮生长因子A(Vascular Endothelial Growth Factor A,VEGFA)。GO分析主要为对无机物的反应、细胞成分运动的正调控、膜伐、质膜蛋白复合物、蛋白质结构域特异性结合、蛋白质同二聚化活性等。KEGG通路富集分析主要涉及癌症途径、脂质和动脉粥样硬化、乙型肝炎、化学致癌-受体激活、肿瘤坏死因子信号通路、化学致癌-活性氧的主要作用。结论:人参-黄芪中的活性成分主要通过AKT1、IL-6、TNF、CASP3、IL-1β、TP53、VEGFA等靶点来调节炎症细胞因子、抗炎、抗氧化,从而达到治疗缺血性心肌病的目的。Objective:To investigate the action mechanism of Renshen(Ginseng)-Huangqi(Astragalus) medicine pair in the treatment of ischemic cardiomyopathy.Methods:The active constituents and targets of Renshen and Huangqi were retrieved by TCMSP.Cy to scape software was used to construct the "medicine-active ingredient-key target" network,and the PPI network was obtained by String platform.The GO enrichment analysis and KEGG pathway enrichment analysis were carried out through Metascape database.Results:The active comstittuents-targets network contained 42 compounds and 718 corresponding targets,with 100 medicine intersection targets.The medicine active ingredients were mainly quercetin kaempferol,7-O-methyl-isomicrocystis sylvatol,formononetin.lsorhamnetin,etc..The key targets of PPI network were AKT1,IL-6,TNF,CASP3,TL-1β,TP53,VEGFA.The GO enrichment analysis mainly involved response to inorganic substances,positive regulation of cellular component movement,membrane depletion,plasma membrane protein complexes,protein domain specific binding,protein homo dimerization activity and so on.The KEGG pathway enrichment analysis mainly involved cancer pathway,lipids and atherosclerosis,hepatitis B,chemical carcinogenesis-receptor activation,tumor necrosis factor signaling pathway,the main role of chemical carcinogenesis-reactive oxygen species,etc.Conclusion:The active components in Renshen-Huangqi medicine pair mainly achieve the purpose of regulating inflammatory cytokines,anti-inflammatory and antioxidant through AKT1,IL-6,TNF,CASP3,IL-1β,TP5 3,VEGFA and other targets,so as to treat ischemic cardiomyopathy.

关 键 词：人参-黄芪 网络药理学 缺血性心肌病 作用机制 靶点 

分 类 号：R542[医药卫生—心血管疾病]