EDI-OCT评估尾部悬吊大鼠视乳头周围视网膜和脉络膜厚度变化  

Assessment of peripapillary retinal and choroid thickness of tail-suspension rats by EDI-OCT

作　　者：赵宏伟 吴斌[2] 阚广捍[2] 张燕[3] 宋飞龙 杨艳艳 黄泽民[2] 王菲 石琳 宫玉波 赵军 罗灵 Zhao Hongwei;Wu Bin;Guanghan Kan;Zhang Yan;Song Feilong;Yang Yanyan;Huang Zemin;Wang Fei;Shi Lin;Gong Yubo;Zhao Jun;Luo Ling(Department of Ophthalmology,Strategic Support Forces Specialized Medical Center,Beijing 100101,China;China Astronaut Research and Training Center,Beijing 100094,China;Department of Ophthalmology,The Seventh Medical Center of Chinese PLA General Hospital,Beijing 100700,China)

机构地区：[1]战略支援部队特色医学中心眼科,北京100101 [2]中国航天员科研训练中心,北京100094 [3]解放军总医院第七医学中心眼科,北京100700

出　　处：《中华航空航天医学杂志》2022年第3期134-139,共6页Chinese Journal of Aerospace Medicine

基　　金：中国航天医学工程预先研究项目(2017SY54A0302,SYFD2018080103)。

摘　　要：目的评估尾部悬吊模拟失重大鼠视乳头周围视网膜和脉络膜厚度的变化。方法 60只大鼠分为对照组(15只)和尾部悬吊(简称尾悬)组(45只)。尾悬组采用-30°尾部悬吊建立模拟失重效应动物模型。分别在尾悬前及尾悬4、8周后采用光学相干断层扫描增强深度成像(enhanced depth imaging-optical coherence tomography, EDI-OCT)测量两组大鼠视乳头周围视网膜厚度;分别在尾悬前及尾悬4、8、10、12周后采用EDI-OCT测量两组大鼠脉络膜厚度。结果尾悬组大鼠在不同时间点视乳头周围视网膜厚度差异有统计学意义(F=30.89, P<0.001), 且随着尾悬时间延长, 其厚度呈下降趋势。尾悬8周后, 尾悬组视乳头周围视网膜厚度与对照组比较差异有统计学意义(t=5.73, P<0.001)。尾悬组大鼠在尾悬4、8、10、12周后脉络膜厚度与对照组比较, 差异均有统计学意义(t=6.32、12.78、9.69、6.39, P值均<0.001);不同时间点尾悬组大鼠脉络膜厚度差异有统计学意义(F=51.08, P<0.001);随着尾悬时间延长, 脉络膜厚度及其与尾悬前的差值均呈上升趋势, 8周后达到峰值, 后略有下降。结论随着尾悬时间延长, 尾悬组大鼠的视乳头周围视网膜厚度总体呈下降趋势;脉络膜厚度变化值总体呈上升趋势, 达到峰值后略有下降。Objective To evaluate the changes of peripapillary retinal and choroid thickness of tail-suspension rats in simulated weightless.Methods Sixty rats were divided into 2 groups:control group(15 rats)and tail-suspension group(45 rats).The rats in tail-suspension group were tested by-30°tail suspension to simulate the effect of weightlessness for establishing model.The peripapillary retinal thickness of the rats were measured before and after 4-and 8-week tail suspension by enhanced depth imaging-optical coherence tomography(EDI-OCT).The choroid thickness of the rats were measured before and after 4-,8-,10-,and 12-week tail-suspension by EDI-OCT.Results There was a significant difference in the peripapillary retinal thickness among the groups with different suspension time(F=30.89,P<0.001),and the thickness showed a downward trend with the extension of the tail suspension time.After 8 weeks of tail-suspension,there was a significant difference in the peripapillary retinal thickness between the tail-suspension group and the control group(t=5.73,P<0.001).After 4,8,10,and 12 weeks of tail-suspension,there were significant differences in the choroidal thickness of the rats between the tail-suspension group and the control group(t=6.32,12.78,9.69,6.39,all P<0.001);there was a significant difference in the choroidal thickness of the rats with different suspension time(F=51.08,P<0.001).With the time of the tail suspension,the choroidal thickness and its changes compared with before tail suspension showed an upward trend,and reached peak after 8 weeks and then slightly decreased.Conclusions With the extension of tail-suspension time,the changes of peripapillary retinal thickness of the tail-suspension rats generally show a downward trend,and the changes of choroidal thickness generally increased,and decreased slightly after reaching peak.

关 键 词：大鼠 失重模拟 视网膜 脉络膜 体层摄影术 光学相干 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]