2016-2021年湖北省乙型Victoria系流感病毒流行病学特征及基因进化分析  

Epidemiological and genetic characteristics of influenza B virus Victoria lineage in Hubei,2016-2021

作　　者：于甜甜 余晓[2] 韩诗 刘琳琳[2] 陈丹[1] Yu Tiantian;Yu Xiao;Han Shi;Liu Linlin;Chen Dan(Department of Nutrition,Hygiene and Toxicology,School of Public Health,Medical College,Wuhan University of Science and Technology,Academy of Nutrition and Health,Wuhan University of Science and Technology,Wuhan 430065,Hubei,China;Institute of Health Inspection and Testing,Hubei Provincial Center for Disease Control and Prevention,Wuhan 430079,Hubei,China)

机构地区：[1]武汉科技大学公共卫生学院营养卫生与毒理学系,武汉科技大学营养与健康研究院,湖北武汉430065 [2]湖北省疾病预防控制中心,湖北武汉430079

出　　处：《疾病监测》2022年第10期1310-1317,共8页Disease Surveillance

基　　金：湖北省科技重大专项(No.2021ACB005)。

摘　　要：目的分析湖北省2016—2021年度乙型流感流行状况及病毒基因进化特征。方法根据湖北省2016—2021年度的流感样病例报告数据和病原学监测数据进行流行病学分析并选取2016—2021年度主要流行的乙型流感毒株69株进行全基因组测序,利用生物信息学软件分析进化变异特征。结果2016—2021年湖北省乙型Victoria系流感毒株小部分分布在Victoria1A分支,而大部分毒株主要集中分布在以162~164氨基酸位点缺失为特征的Victoria 1A.3分支上。与参考株相比,流行株在HA蛋白的120环、150环、160环及190螺旋等关键的抗原决定簇部位发生了多个氨基酸位点改变,而在NA蛋白上未发现神经氨酸酶抑制剂耐药位点的变异。本次研究仅发现1株系内重配株。结论2016—2021年中湖北省乙型流感多为季节性流行,Victoria系流行毒株在多个抗原位点上发生变异,但未检测到耐药位点突变,因此需要进一步密切加强流感流行活动的监测。Objective To analyze the epidemiological and genetic characteristics of influenza B virus in Hubei province from 2016 to 2021.Methods Based on the reported incidence data of influenza-like illness(ILI)and the etiological surveillance results in Hubei during 2016-2021,69 strains of influenza B virus were selected to perform whole genome sequencing and bioinformatics software was used to analyze the evolutionary variation characteristics.Results During 2016-2021,a small proportion of influenza B virus Victoria lineage strains in Hubei belonged to the Victoria 1A branch,most strains belonged to the Victoria 1A.3 branch,which is characterized by deletion of amino acid sites 162–164.Compared with the reference strain,the circulating strain showed multiple amino acid site changes in the key antigenic determinant clusters of the HA protein at the 120 loop,150 loop,160 loop and 190 helix,but no neuraminidase inhibitor resistance site variants were found on the NA protein.Only 1 reassortment strain was found in this study.Conclusion Influenza B is mostly seasonal in Hubei province during 2016 to 2021,there were multiple antigenic site mutations in epidemic strains of the Victoria B lineage,however,no mutations in the drug resistance sites were found and need to strengthen influenza surveillance.

关 键 词：乙型流感 Victoria系 同源性分析 基因序列分析 

分 类 号：R211[医药卫生—中医学] R373.1