基于基因编辑技术的非人灵长类动物模型构建  

Construction of non-human primate models based on gene editing technology

作　　者：陈红玉 刘真 孙强[1] CHEN Hong-Yu;LIU Zhen;SUN Qiang(State Key Laboratory of Neuroscience,Key Laboratory of Primate Neurobiology,CAS Center for Excellence in Brain Science and Intelligence Technology,Institute of Neuroscience,Chinese Academy of Sciences,Shanghai 200031,China)

机构地区：[1]中国科学院神经科学研究所,中国科学院脑科学与智能技术卓越创新中心,神经生物学国家重点实验室,中国科学院灵长类神经生物学重点实验室,上海200031

出　　处：《生命科学》2022年第10期1240-1249,共10页Chinese Bulletin of Life Sciences

基　　金：国家自然科学基金项目(31825018,82021001,2021ZD020900);中国科学院战略性先导科技专项(B类)(XDB32060100);临港实验室重点任务资助项目(LG202106-01-01,LG202106-02-01)。

摘　　要：非人灵长类疾病模型在生命科学和生物医药领域具有非常重要的地位。近年来,随着经典的靶向核酸酶(ZFNs、TALENs和CRISPR/Cas9)基因编辑技术的出现,科学家已经成功获得了多种人类疾病相关的非人灵长类模型。该文首先介绍了经典基因编辑技术和以此为基础开发的单碱基编辑(base editing,BE)以及先导编辑(prime editing,PE)技术的研究进展,随后对近年来通过不同基因编辑技术获得的非人灵长类疾病模型进行了综述,最后对目前已获得的和正在构建的非人灵长类模型的管理、保种以及动物伦理和福利等方面进行了展望。Non-human primate disease models have a very important position in the fields of life sciences and biomedicine.In recent years,with the advent of classical targeted nuclease(ZFNs,TALENs,and CRISPR/Cas9)gene editing technologies,scientists have successfully obtained a variety of human disease-related non-human primate models.This paper firstly introduces the research progress of classical gene editing technology and single base editing(BE)and prime editing(PE)technology developed based on CRISPR/Cas9,subsequently,the nonhuman primate disease models obtained by different gene editing techniques in recent years were reviewed,and finally,the management,conservation,animal ethics and welfare of the non-human primate models that have been obtained and are being constructed are prospected.

关 键 词：非人灵长类动物模型 基因编辑 CRISPR/Cas9 单碱基编辑(BE) 先导编辑(PE) 

分 类 号：Q78[生物学—分子生物学]