水淹胁迫对甘薯生长和养分分配的影响  

Effects of waterlogging stress on growth and nutrient distribution of sweet potato

作　　者：王芳[1,2] 宋伟 杨松涛 乔帅[1,2] 廖安忠 谭文芳 WANG Fang;SONG Wei;YANG Songtao;QIAO Shuai;LIAO Anzhong;TAN Wenfang(Crop Research Institute,Sichuan Academy of Agricultural Sciences,Chengdu 610066,China;Environment-friendly Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province,Chengdu 610066,China)

机构地区：[1]四川省农业科学院作物研究所,成都610066 [2]粮油作物绿色种质创新与遗传改良四川省重点实验室,成都610066

出　　处：《植物生理学报》2022年第10期1864-1872,共9页Plant Physiology Journal

基　　金：国家现代农业产业技术体系(CARS-10-B04);四川省农业农村厅重点研发项目(2021YFYZ0019)。

摘　　要：水淹胁迫是制约甘薯产量最重要的非生物胁迫之一。然而,甘薯响应水淹胁迫的生理基础及分子机制目前研究较少。以三个对水淹胁迫具有不同敏感性的甘薯栽培品种‘广薯87’、‘川紫薯6号’和‘南薯88’为研究对象,采用盆栽土培方法,进行长时间水淹和非水淹处理。30 d后统计水淹胁迫对甘薯块根膨大、生物量以及养分氮、磷、钾分配的影响,解析甘薯在水淹胁迫下的生理变化。表型结果显示长时间水淹胁迫能够促进甘薯地上部分的伸长生长,抑制块根的膨大。养分含量测定结果显示,水淹胁迫通过加速氮、磷、钾营养元素向地上部分分配,促进地上部生长进而逃逸水淹胁迫。以已测序的甘薯栽培品种‘泰中6号’进行RT-qPCR分析,结果显示水淹胁迫能够诱导甘薯氮、磷、钾转运体、乙烯合成酶(IbACS1)和淀粉酶(IbAmyase1)的基因表达,提示在水淹胁迫下乙烯、淀粉酶途径以及养分相关的关键基因可能共同参与到甘薯水淹胁迫信号途径,这为进一步深入研究甘薯水淹胁迫的机制提供参考。Waterlogging stress is one of the main abiotic stresses limiting sweet potato production.However,the physiological basis and molecular mechanism of sweet potato response to waterlogging stress remain unclear.In this study,three sweet potato varieties‘Guangshu87’,‘Chuanzishu6’and‘Nanshu88’,based on the different sensitivity to waterlogging stress,were planted in pot with waterlogging stress treatment and normal watering treatment.After 30 days,the effect of waterlogging stress on the biomass,bulking of root tube and the content of nitrogen,phosphorus and potassium were analysed,and these results uncovered the influence of physiological changes of sweet potato under waterlogging stress.The phenotypic analysis showed that sustained waterlogging stress promoted the growth of shoot,but root tube formation and bulking were inhibited.The nutrient content analysis indicated that waterlogging stress promoted the distribution of nitrogen,phosphorus and potassium to the shoot and accelerated the shoot growth to escape from waterlogging stress.RT-qPCR analysis by the sequenced variety‘Taizhong6’showed that waterlogging stress induced the expression of genes encoding nutrient transporters,ethylene synthase(IbACS1)and starch synthase(IbAmyase1).These results suggested that ethylene,amylase pathways and key genes related to nutrients may be involved in the signaling pathway of waterlogging stress in sweet potato.This study provides reference for further study on the waterlogging stress mechanism of sweet potato.

关 键 词：甘薯 水淹胁迫 养分分配 乙烯合成酶 淀粉酶 

分 类 号：S531[农业科学—作物学]