hESC衍生和组织源MSC标记GFP基因的特性分析  

Characterization of hESC-derived and Adult Mesenchymal Stem Cells After Labeling the GFP Gene

作　　者：刘云[1] 叶进培 郭兴萍 LIU Yun;YE Jinpei;GUO Xingping(Department of Biochemistry and Molecular Biology,Shanxi Medical University,Taiyuan 030001,China;Institute of Biomedicine,Shanxi University,Taiyuan 030006,China;Shanxi Bethune Hospital,Shanxi Academyof Medical Sciences,Taiyuan 030032,China)

机构地区：[1]山西医科大学生物化学与分子生物学教研室,山西太原030001 [2]山西大学生物医学研究院,山西太原030006 [3]山西白求恩医院(山西医学科学院),山西太原030032

出　　处：《山西大学学报（自然科学版）》2022年第5期1402-1410,共9页Journal of Shanxi University(Natural Science Edition)

摘　　要：干细胞标记示踪在研究细胞治疗机理中具有重要作用,而标记物对干细胞的安全可靠性是细胞标记示踪的重要前提。文章对人胚胎干细胞衍生的间充质干细胞(hESC-MSC)、人脂肪间充质干细胞(hADSC)和人脐带间充质干细胞(hUC-MSC)进行了绿色荧光蛋白(GFP)基因慢病毒转导并比较它们在转导前后的增殖、多项分化潜能和细胞表型等生物学特性以及它们的转导效率。结果表明,在等量pLVX-IRES-Puro-GFP慢病毒载体转导下,hESC-MSC细胞慢病毒转导效率显著高于h ADSC(P<0.01)和hUC-MSC(P<0.01);但是hESC-MSC、hADSC和h UC-MSC标记GFP基因前后细胞增长形态、增殖力、细胞表面抗原的表达和分化潜能均没有明显差异。文章提示不同来源的间充质干细胞均可进行安全可靠的GFP基因标记,而人胚胎干细胞衍生的间充质干细胞可能更适合用以慢病毒感染的示踪标记或者基因修饰。A safe and reliable labeling for stem cells is important for the study on mechanism of cell therapy.The purpose of this study was to establish a p LVX-IRES-Puro-GFP lentiviral transduction for human embryonic stem cell-derived mesenchymal stem cell(hESC-MSC),human adipose-derived MSC(hADSC)and human umbilical cord-derived MSC(hUC-MSC),and to analyze any difference in characteristics pre-and post-labelling with GFP gene.The results showed that hESC-MSC,hADSC and hUC-MSC all had no alteration in standard characterization including cell morphology,the expression of cell surface antigens and trilineage differentiation potential.However,the lentivirus transduction efficiency for hESC-MSC was significantly higher than hADSC(P<0.01)and hUC-MSC(P<0.01).GFP labelling via lentivirus is a reliable and safe labeling approach for these three MSCs and hESC-MSC might be better suitable for labelling via lentivirus and gene modifications.

关 键 词：人胚胎干细胞(hESC) 间充质干细胞(MSC) 绿色荧光蛋白(GFP) 慢病毒 

分 类 号：Q26[生物学—细胞生物学]