羌药验方制剂接骨丸质量标准研究  

Study on quality standard of Qiang medicine prescription Jiegu pill

作　　者：郭欣悦 刘莹 庞欣怡 于畅 张力丹 王战国[3] 李云丹 范维强 GUO Xin-yue;LIU Ying;PANG Xin-yi;YU Chang;ZHANG Li-dan;WANG Zhan-guo;LI Yun-dan;FAN Wei-qiang(School of Food and Biological Engineering,Chengdu University,Chengdu 610106,Sichuan;School of pharmacy,Chengdu University,Chengdu 610106,Sichuan;School of Preclinical Medicine,Chengdu University,Chengdu 610106,Sichuan;Aba State Food and Drug Inspection Institute,Maerkang 624000,Sichuan)

机构地区：[1]成都大学食品与生物工程学院,成都610106 [2]成都大学药学院,成都610106 [3]成都大学基础医学院,成都610106 [4]阿坝州食品药品检验中心,马尔康624000

出　　处：《中药与临床》2022年第4期13-16,共4页Pharmacy and Clinics of Chinese Materia Medica

基　　金：四川省科技厅项目(2019YFN0127,2019ZYD052);四川省科技厅平台搭建项目(2019JDPT0019);大学生创新训练计划项目(S202111079143S,202011079023)。

摘　　要：目的:建立羌药验方制剂接骨丸的质量控制方法。方法:采用薄层色谱法鉴别方中白术,姜黄;采用HPLC测定接骨丸中姜黄素的含量,色谱柱为SWELL Chromplus-C18色谱柱(4.6×250 mm,5μm),流动相为乙腈-0.4%冰醋酸溶液(50∶50,v/v),检测波长为430 nm。结果:薄层色谱鉴别方法专属性强,斑点清晰,且阴性无干扰;姜黄素在1.67~20μg·mL^(-1)范围内呈良好的线性关系。接骨丸中姜黄素平均含量为0.59 mg·g^(-1)。结论:建立的薄层色谱鉴别和HPLC可用于接骨丸的质量控制。Objective: To establish a method for the quality control of Qiang medicine prescription Jiegu pill. Method: The TLC method was used to identify Baizhu and Jianghuang in the prescription. The HPLC method was used to detect the content of curcumin in Jiegu pill on SWELL Chromplus-C18 chromatographic column(4.6×250 mm, 5 μm). The mobile phase was acetonitrile-0.4% glacial acetic acid solution(50:50, V/V), and the detection wavelength was 430 nm. Result: The TLC method was highly specific, with clear spots and no interference of the negative control. Curcumin showed a good linearity in the range of 1.67~20.00 μg·mL^(-1). The average content of curcumin in Jiegu pill was 0.59 mg·g^(-1). Conclusion: The established TLC and HPLC can be used for the quality control of Jiegu pill.

关 键 词：接骨丸 姜黄素 薄层鉴别 高效液相色谱法 质量标准 

分 类 号：R283.1[医药卫生—中药学]