川崎病患儿血浆miR-26b水平与冠状动脉损伤的相关性  

Correlation between Plasma miR-26b Level and Coronary Artery Injury in Children with Kawasaki Disease

作　　者：朱其菲 孙锋 ZHU Qi-fei;SUN Feng(Ankang Central Hospital,Ankang Shaanxi 725000)

机构地区：[1]安康市中心医院儿科,陕西安康725000 [2]安康市中心医院新生儿科,陕西安康725000

出　　处：《医学临床研究》2022年第11期1708-1711,共4页Journal of Clinical Research

摘　　要：【目的】探讨川崎病患儿血浆微小RNA-26b(miR-26b)与冠状动脉损伤的相关性。【方法】选择2018年2月至2020年7月本院收治的106例川崎病患儿(观察组),根据冠状动脉损伤情况将其分为损伤组(n=34)和无损伤组(n=72)。同时,选择同期在本院体检的110例健康儿童作为对照组。采用实时荧光定量聚合酶链式反应(qRT-PCR)检测血浆miR-26b表达水平,Pearson分析观察组血浆中miR-26b与血管内径关系,绘制受试者工作特征(ROC)曲线分析血浆中miR-26b水平对川崎病患儿冠状动脉损伤情况的预测价值。【结果】观察组血浆miR-26b表达水平为0.69±0.12,显著低于对照组的1.02±0.16,差异有统计学意义(t=17.10,P<0.05)。Pearson相关性分析结果显示,血浆中miR-26b与血管内径呈负相关(r=-0.410,P<0.05)。ROC曲线显示,血浆miR-26b水平预测川崎病患儿冠状动脉损伤的ROC曲线下面积为0.807(95%CI:0.724~0.890),截断值为0.69,敏感度为85.30%、特异性为70.80%。【结论】川崎病患儿血浆miR-26b呈低表达,且川崎病冠状动脉损伤患儿中miR-26b水平更低,与血管内径呈负相关,血浆miR-26b对冠状动脉损伤有一定预测价值。【Objective】To investigate the relationship between plasma microRNA-26b(miR-26b)and coronary artery injury in children with Kawasaki disease.【Methods】A total of 106 children with Kawasaki disease admitted to our hospital from February 2018 to July 2020(observation group)were selected and divided into injury group(n=34)and non injury group(n=72)according to the condition of coronary artery injury.At the same time,110 healthy children in the same period were selected as the control group.The expression level of miR-26b in plasma was detected by real-time fluorescence quantitative polymerase chain reaction(qRT-PCR),and the relationship between miR-26b and vascular diameter in plasma of patients was analyzed by Pearson;The predictive value of miR-26b level in plasma for coronary artery injury in children with Kawasaki disease was analyzed by drawing the receiver operating characteristic(ROC)curve.【Results】The expression level of miR-26b in plasma of the observation group was 0.69±0.12,which was significantly higher than that of the control group(1.02±0.16),and the difference was statistically significant(t=17.10,P<0.05).Pearson correlation analysis showed that there was a correlation between miR-26b in plasma and vessel diameter in the diseased group.The results showed that there was a negative correlation between miR-26b in plasma and vessel diameter(r=-0.410,P<0.05).The ROC curve showed that the area under the ROC curve of plasma miR-26b level predicting coronary artery injury in children with Kawasaki disease was 0.807(95%CI:0.724~0.890),the cut-off value was 0.69,the sensitivity was 85.30%,and the specificity was 70.80%.【Conclusion】The expression of miR-26b in plasma is low in children with Kawasaki disease,and the level of miR-26b is lower in children with coronary artery injury in Kawasaki disease,which is negatively related to the diameter of blood vessels.The plasma miR-26b has certain value in predicting coronary artery injury.

关 键 词：黏膜皮肤淋巴结综合征 微RNAS 冠状动脉疾病 

分 类 号：R72[医药卫生—儿科]