cGMP蛋白激酶1在子痫前期中的表达及其对子宫内膜间质细胞蜕膜化与滋养细胞侵袭的影响  

Expression of PKG1 in preeclampsia and its effect on decidualization of endometrial stromal cells and invasion of trophoblasts

作　　者：郭婷[1] 孙一 王海丽 郑雪绒 GUO Ting;SUN Yi;WANG Haili;ZHENG Xuerong(Second Department of Obstetrics,Second Affiliated Hospital of Xi′an Medical College,Xi′an 710038,Shaanxi,China;Department of Obstetrics and Gynecology,Chang′an Hospital,Xi′an 710061,Shaanxi,China;Department of Obstetrics,Shaanxi Provincial People′s Hospital,Xi′an 710054,Shaanxi,China)

机构地区：[1]西安医学院第二附属医院产二科,西安710038 [2]长安医院妇产科,西安710061 [3]陕西省人民医院产一科,西安710054

出　　处：《中国性科学》2022年第12期85-89,共5页Chinese Journal of Human Sexuality

基　　金：陕西省重点研发计划项目(2019SF-092)。

摘　　要：目的探究cGMP蛋白激酶1(PKG1)在子痫前期(PE)中的表达及其对子宫内膜间质细胞(hESCs)蜕膜化和滋养细胞侵袭能力的影响。方法选取2019年2月至2021年2月于西安医学院第二附属医院产二科住院分娩的25例PE患者(PE组)和25例健康妊娠者(Normal组)作为研究对象,采用实时荧光定量聚合酶链反应(RT-PCR)和免疫印迹(Western blot)法检测两组孕产妇胎盘蜕膜组织中PKG1 mRNA与蛋白的表达。体外培养hESCs,采用二丁酰环磷酸腺苷(cAMP)与醋酸甲羟孕酮-17-乙酸酯(MPA)联合诱导使hESCs发生蜕膜化,观察蜕膜化细胞的形态学改变;采用RT-PCR检测蜕膜化细胞中催乳素(PRL)和胰岛素生长因子结合蛋白(IGFBP1)的mRNA表达;采用RT-PCR、Western blot法检测PKG1在蜕膜化hESCs中的表达。将si-PKG1转染入hESCs中,并将细胞分为三组:对照组、cAMP+MPA+si-NC组与cAMP+MPA+si-PKG1组;采用RT-PCR检测各组hESCs中PRL、IGFBP1和血管内皮生长因子(VEGF)mRNA的表达;采用Transwell法检测各组hESCs对滋养层细胞系HTR-8侵袭与迁移功能的影响。结果与Normal组相比,PKG1在PE组中的表达降低(P<0.01)。hESCs经cAMP与MPA诱导后发生典型蜕膜样改变,同时细胞中PRL、IGFBP1和PKG1的表达增加(P<0.05);与对照组相比,cAMP+MPA+si-NC组和cAMP+MPA+si-PKG1组中hESCs呈明显蜕膜化改变,细胞中PRL、IGFBP1和VEGF mRNA表达升高(P<0.01),HTR-8细胞的侵袭与迁移能力增强(P<0.05),但与cAMP+MPA+si-NC组相比,cAMP+MPA+si-PKG1组的上述检测指标的变化趋势均降低(P<0.05)。结论PE患者胎盘蜕膜组织中PKG1表达降低,其可能通过影响hESCs的蜕膜化过程抑制血管生成与滋养细胞的侵袭,从而参与PE的发生发展。Objective To investigate the expression of cGMP protein kinase 1(PKG1)in preeclampsia(PE)and its effect on decidualization of human intrauterine interstitial cells(hESCs)and invasion of trophoblast cells.Methods A total of 25 PE patients(PE group)and 25 healthy pregnant people(Normal group)who delivered in the Second Department of Obstetrics,Second Affiliated Hospital of Xi′an Medical College,from February 2019 to February 2021 were selected as the research objects.Realtime fluorescence quantitative polymerase chain reaction(RT-PCR)and Western blot were used to detect the expression of PKG1 mRNA and protein in the decidua of placenta in both groups.hESCs were cultured in vitro.And the decidualization of hESCs was induced by dibutyl cyclic adenosine monophosphate(cAMP)and medroxyprogesterone-17-acetate(MPA).The morphological changes of decidualized cells were observed.The mRNA expressions of prolactin(PRL)and insulin growth factor binding protein(IGFBP1)in deciduated cells were detected by RT-PCR.The expression of PKG1 in decidualized hESCs was detected by RT-PCR and Western blot.hESCs was transfected with si-PKG1 and cells were divided into 3 groups:control group,cAMP+MPA+si-NC group and cAMP+MPA+si-PKG1 group.RT-PCR was used to detect PRL and IGFBP1 in hESCs and the mRNA expression of vascular endothelial growth factor(VEGF).Transwell assay was used to detect the effects of hESCs on the invasion and migration of trophoblast cell line HTR-8.Results Compared with Normal group,the expression of PKG1 in PE group was significantly decreased(P<0.01).hESCs were induced by cAMP and MPA,and the expression of PRL,IGFBP1 and PKG1 was increased(P<0.05).Compared with the control group,hESCs in cAMP+MPA+si-NC group and cAMP+MPA+si-PKG1 group showed obvious decidualization changes,and mRNA expressions of PRL,IGFBP1 and VEGF were increased(P<0.01).The invasion and migration ability of HTR-8 cells were enhanced(P<0.05),but compared with cAMP+MPA+si-NC group,the change trend of the above indexes in cAMP+MPA+si-PKG1 group was de

关 键 词：子痫前期 子宫内膜间质细胞 cGMP蛋白激酶1 滋养细胞 

分 类 号：R714[医药卫生—妇产科学]