针刺治疗对抽动模型大鼠儿茶酚胺类降解酶和多巴胺受体的影响  

Effects of Acupuncture on Catecholamine-degrading Enzymes and Dopamine Receptors in Twitch Model Rats

作　　者：方春凤 潘静巧 李丹英 狄朋桃 FANG Chunfeng;PAN Jingqiao;LI Danying;DI Pengtao(Department of Pediatrics,the First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine/Yunnan Provincial Hospital of Traditional Chinese Medicine,Yunnan Province,Kunming 650021,China;Department of Pediatrics,Chuxiong Prefecture Hospital of Traditional Chinese Medicine/Yunnan Yi Medical Hospital,Yunnan Province,Chuxiong 675000,China;Department of Neurosurgery,Yuxi People’s Hospital,Yunnan Province,Yuxi 653300,China;Department of Rheumatology,the First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine/Yunnan Provincial Hospital of Traditional Chinese Medicine,Yunnan Province,Kunming 650021,China)

机构地区：[1]云南中医药大学第一附属医院/云南省中医医院儿科,云南昆明650021 [2]楚雄州中医医院/云南省彝医医院儿科,云南楚雄675000 [3]玉溪市人民医院神经外科,云南玉溪653300 [4]云南中医药大学第一附属医院/云南省中医医院风湿科,云南昆明650021

出　　处：《中国中医药现代远程教育》2023年第2期161-164,共4页Chinese Medicine Modern Distance Education of China

基　　金：云南省卫生科技计划项目【No.2018NS0030】。

摘　　要：目的观察针刺对抽动障碍模型大鼠脑中儿茶酚胺类降解酶COMT、MAO-B和多巴胺受体D1、D2表达的影响。方法实验大鼠造模成功后随机分为模型组、针刺治疗组、对照组,同时设立空白组正常饲养,每组10只。治疗组予针刺治疗,对照组予氟哌啶醇片灌胃,空白组与模型组给予等体积生理盐水灌胃,治疗4周。治疗结束后取脑组织,检测COMT、MAO-B、DRD1、DRD2的mRNA表达量。结果治疗4周后,与模型组和对照组比较,针刺组大鼠脑中COMT、MAO-B的mRNA表达量均明显升高(P<0.05);与模型组比较,针刺组大鼠脑中DRD1的mRNA表达量明显降低,DRD2的mRNA表达量明显升高(P<0.05)。结论针刺治疗能通过影响IDPN模型大鼠脑中COMT、MAO-B、DRD1、DRD2的表达水平达到治疗抽动障碍的作用。Objective To observe the effects of acupuncture on the expression levels of catecholamine-degrading enzymes COMT,MAO-B and dopamine receptors D1 and D2 in brain of tic disorder(TD)model rats.Methods After successful modeling,experimental rats were randomly divided into model group,acupuncture group and control group.At the same time,blank group was set up to feed normally,with 10 rats in each group.The treatment group was treated with acupuncture,the control group was given haloperidol by gavage,and the blank group and model group were given equal volume pure water for 4 weeks.After treatment,brain tissues were taken to detect the mRNA expression levels of COMT,MAO-B,DRD1 and DRD2.Results After 4 weeks of treatment,compared with model group and control group,the mRNA expression levels of COMT and MAO-B in brain of acupuncture group were significantly increased(P<0.05).Compared with model group,the mRNA expression level of DRD1 was significantly decreased,while the mRNA expression level of DRD2 was significantly increased(P<0.05).Conclusion Acupuncture therapy can treat tic disorder by affecting the contents of COMT,MAO-B,DRD1 and DRD2 in the brain of IDPN model rats.

关 键 词：抽动障碍 针刺疗法 大鼠 

分 类 号：R245[医药卫生—针灸推拿学]