"推髌伸膝"手法对兔膝骨性关节炎模型关节软骨ITGβ1与p-FAK表达的影响  

Effect of"patellar pushing and knee extension"manipulation on integrinβ1 and p-FAK in articular cartilage of rabbit model of knee osteoarthritis

作　　者：李远栋[1] 张超[1] 苏瑾[1] 符碧峰 刘爱峰[1] 王平[1] Li Yuandong;Zhang Chao;Su Jin;Fu Bifeng;Liu Aifeng;Wang Ping(Orthopedics and Traumatology,First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion,Tianjin 300381,China)

机构地区：[1]天津中医药大学第一附属医院骨伤科,国家中医针灸临床医学研究中心,天津300381

出　　处：《国际生物医学工程杂志》2022年第2期99-103,共5页International Journal of Biomedical Engineering

基　　金：天津市教委科研计划项目(2019KJ064)。

摘　　要：目的观察"推髌伸膝"手法对兔膝骨性关节炎(KOA)模型关节软骨整合素β1(ITGβ1)与磷酸化黏着斑激酶(p-FAK)的蛋白和mRNA表达水平的影响,探讨手法治疗KOA的作用机制。方法将20只健康6月龄新西兰家兔,按随机数字表法分为正常组、模型组、针刺组及手法组。其中,模型组、针刺组与手法组使用改良后的Hulth法进行KOA造模。造模成功7 d后,进行试验。正常组与模型组不予任何干预,针刺组和手法组每天分别接受1次针刺干预和"推髌伸膝"手法干预。治疗2周后,兔KOA模型以空气栓塞法处死,分别采用Western blot和实时聚合酶链式反应(PCR)法检测膝关节软骨ITGβ1和p-FAK的蛋白和mRNA表达水平。结果与正常组比较,模型组的ITGβ1蛋白表达水平降低(P<0.05),p-FAK蛋白表达水平升高(P<0.05),ITGβ1及p-FAK的mRNA表达水平无明显变化(均P>0.05);与模型组比较,针刺组的ITGβ1蛋白表达水平升高(P<0.05),p-FAK蛋白表达水平降低(P<0.05),ITGβ1及p-FAK的mRNA表达水平均升高(均P<0.05);与针刺组比较,手法组的ITGβ1蛋白表达水平升高(P<0.05),p-FAK蛋白表达水平降低(P<0.05),ITGβ1及p-FAK的mRNA表达水平均升高(均P<0.01)。结论"推髌伸膝"手法能改善兔KOA模型关节软骨ITGβ1和p-FAK的蛋白和mRNA表达水平据。Objective To observe the effects of"pushing patella and extending knee"manipulation on the protein and mRNA expression levels of integrinβ1(ITGβ1)and phosphorylated adhesion plaque kinase(p-FAK)in rabbit knee osteoarthritis(KOA)model,and to investigate the mechanism of manipulations in the treatment of KOA.Methods Twenty healthy 6-month-old New Zealand rabbits were divided into the normal group,the model group,the acupuncture group,and the manipulation group according to the random number table method.Among them,the model group,the acupuncture group,and the manipulation group were modeled using the modified Hulth method for KOA.After 7 d of successful modeling,the normal group and the model group did not receive any intervention,while the acupuncture group and the manipulation group received one acupuncture intervention and one"pushing patella and extending knee"manipulation intervention daily,respectively.After 2 weeks of treatment,the rabbit KOA model was executed by air embolization,and the protein and mRNA expression levels of ITGβ1 and p-FAK in knee cartilage were measured by Western blot and real-time polymerase chain reaction(PCR),respectively.Results Compared with the normal group,the ITGβ1 protein expression level was decreased(P<0.05)and p-FAK protein expression level was increased(P<0.05),and the mRNA expression levels of ITGβ1 and p-FAK did not change significantly(all P>0.05)in the model group.Compared with the model group,the ITGβ1 protein expression level was increased(P<0.05),the p-FAK protein expression level decreased(P<0.05),and the mRNA expression levels of both ITGβ1 and p-FAK increased(all P<0.05)in the acupuncture group.Compared with the acupuncture group,ITGβ1 protein expression level increased(P<0.05),p-FAK protein expression level decreased(P<0.05),and mRNA expression levels of both ITGβ1 and p-FAK increased(all P<0.01)in the manipulation group.Conclusions The"pushing patella and extending knee"manipulation can optimize the protein and mRNA expression levels of ITGβ1 and p-FAK

关 键 词：膝骨性关节炎 "推髌伸膝"手法 整合素Β1 磷酸化黏着斑激酶 

分 类 号：R244.1[医药卫生—针灸推拿学]