激素模拟妊娠后激素停撤大鼠产后抑郁模型的改进与评价  

Improvement and Evaluation of a Rat Model of Postpartum Depression Induced by Hormone Withdrawal Following Hormone-simulated Pregnancy

作　　者：王晓菲 严语 赵展 何沐 王璟萱 武军驻[3] 杨怀洁[4] WANG Xiaofei;YAN Yu;ZHAO Zhan;HE Mu;WANG Jingxuan;WU Junzhu;YANG Huaijie(Renmin Hospital of Wuhan University,Wuhan 430060,China;Zhongnan Hospital of Wuhan University,Wuhan 430071,China;Center for Experimental Basic Medical Education of Wuhan University,Wuhan 430000,China;the People’s Hospital of China Three Gorges University,Yichang 443000,China)

机构地区：[1]武汉大学人民医院,武汉430060 [2]武汉大学中南医院,武汉430071 [3]武汉大学基础医学实验教学中心,武汉430000 [4]三峡大学人民医院,宜昌443000

出　　处：《实验动物科学》2022年第4期13-17,共5页Laboratory Animal Science

基　　金：国家自然科学基金青年基金(81701484)。

摘　　要：目的改进激素模拟妊娠后激素停撤大鼠产后抑郁模型的建立方法,并对模型进行评价。方法25只SD雌性大鼠随机分为正常组(10只)和实验组(15只)。实验组去势手术后筛选合格动物,前16 d每日皮下注射雌二醇2.5μg,黄体酮4 mg,后7 d每日注射雌二醇注射液50μg。正常组不做手术处理,23 d持续注射等量橄榄油。建模后第1周通过旷场实验、强迫游泳实验进行大鼠行为学检测。结果与正常组相比,实验组旷场实验水平运动距离减短,强迫游泳实验不动时间延长,差异均有统计学意义(P<0.05)。结论通过改进建模方法,成功建立稳定的产后抑郁大鼠模型,并探寻到可靠的评价方法。Objective To improve the method of establishing a postpartum depression rat model after hormone withdrawal following hormone-simulated"pregnancy"and evaluate the model.Method 25 SD female rats were randomly divided into normal group(n=10),experimental group(n=15).Qualified animals were selected after ovariectomizing bilaterally in the experimental group,which received daily injections of the hormones estradiol 2.5μg and progesterone 4 mg for the first 16 days,estradiol 50μg for the next 7 days.In the normal group,no treatment was performed,and the same amount of olive oil was injected for 23 days.In the first week after modeling,the behavioral tests were conducted through Open Field Test(OFT)and Forced Swim Test(FST).Result Compared with the normal group,the experimental group exhibits significantly shortened horizontal movement distance in the OFT and increased immobility time in the FST.The difference was statistically significant(P<0.05).Conclusion By improving the method of establishing model,a model of postpartum depression rat after hormone withdrawal following hormone-simulated"pregnancy"was successfully established,and a reliable evaluation method was found.

关 键 词：产后抑郁 动物模型 改良 行为学检测 

分 类 号：Q95-3[生物学—动物学]