不饱和脂肪酸对肝癌移植瘤小鼠P21、P53、P13K及PKB/Akt蛋白表达的影响  

Effects of Unsaturated Fatty Acids on the Expression of P21,P53,P13K and PKB/Akt in Liver Cancer Transplanted Mice

作　　者：朱喜春 张永兴[2] 俞发荣 ZHU Xichun;ZHANG Yongxing;YU Farong(Gansu Institute for Drug Control,Lanzhou 730070,China;Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,State Key Laboratory of Veterinary Etiological Biology,Lanzhou 730046,China;School of Public Security,Gansu University of Political Science and Law,Lanzhou 730070,China)

机构地区：[1]甘肃省药品检验研究院,兰州730070 [2]中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,兰州730046 [3]甘肃政法大学公安分院,兰州730070

出　　处：《实验动物科学》2022年第4期33-38,共6页Laboratory Animal Science

基　　金：兰州市科技计划项目(2019-1-48)。

摘　　要：目的建立SCID小鼠肿瘤模型,为筛选新的抗肿瘤药物提供受试对象。方法将肝癌组织移植到重症联合免疫缺陷(SCID)小鼠皮下,待移植瘤生长到6~7 mm时,50只荷瘤SCID小鼠分为不饱和脂肪酸低、中、高剂量组【50 mg/(kg·d)、100/(kg·d)、200 mg/(kg·d)】,五氟尿嘧啶(5-FU)组【100 mg/(kg·d)】,模型组【蒸馏水2 mL/(kg·d)】,连续给药21 d,剥取移植瘤测量其体质量和体积,比较肿瘤生长情况;用酶联免疫吸附法(ELISA)检测瘤组织P21、P53、P13K、PKB/Akt蛋白表达水平。结果不饱和脂肪酸低、中、高剂量组和5-FU组肝癌移植瘤质量或体积显著小于模型组(P<0.01);肝癌移植瘤P21水平较模型组明显升高(P<0.05,P<0.01);P53、P13K、PKB/Akt水平较模型组明显降低(P<0.05,P<0.01)。结论不饱和脂肪酸对肝癌移植瘤生长具有明显的抑制作用。其可能机制与上调移植瘤P21水平,下调P53、P13K、PKB/Akt水平,抑制PI3K-Akt信号传导通路活性有关。Objective To establish SCID mice tumor model for screening new antitumor drugs.Method Liver cancer tissue was transplanted subcutaneously into severe combined immune deficiency(SCID)mice.When the transplanted tumor grew to 6-7 mm,fifty SCID tumor-bearing mice were divided into unsaturated fatty acid low,medium and high dose groups[50 mg/(kg·d),100/(kg·d),200 mg/(kg·d)].Pentafluorouracil(5-FU)group[100 mg/(kg·d)]and model group[distilled water 2 mL/(kg·d)]were administered continuously for 21 d.The transplanted tumor was removed to measure its quality and volume,and the tumor growth was compared.The protein expression levels of P21,P53,P13 K and PKB/Akt in tumor tissues were determined by enzyme-linked immunosorbent assay(ELISA).Result The weight or volume of liver cancer transplanted tumor in low,medium and high dose of unsaturated fatty acids groups and 5-FU group was significantly lower than that in model group(P<0.01).The level of P21 was significantly higher compared with model group(P<0.05,P<0.01),the levels of P53,P13 K and PKB/Akt were significantly lower than those in model group(P<0.05,P<0.01).Conclusion Unsaturated fatty acids can inhibit the growth of liver cancer transplanted tumor,The possible mechanism is related to up-regulation of P21 level,down-regulation of P53,P13 K,PKB/Akt levels,and inhibition of PI3 K-Akt signal transduction pathway activity.

关 键 词：肝癌动物模型 不饱和脂肪酸 肝癌移植瘤 重症联合免疫缺陷小鼠 

分 类 号：R95-3[医药卫生—药学]