不同遗传背景小鼠输卵管移植效率的研究  

Study on the Efficiency of Tubal Transplantation in Mice with Different Genetic Backgrounds

作　　者：杜淼 陈芳华 战大伟 DU Miao;CHEN Fanghua;ZHAN Dawei(SPF(Beijing)Biotechnology Co.Ltd,Beijing 102101,China)

机构地区：[1]斯贝福(北京)生物技术有限公司,北京102101

出　　处：《实验动物科学》2022年第4期44-46,共3页Laboratory Animal Science

摘　　要：目的选用不同遗传背景的小鼠,研究移植不同胚胎数量对产仔率的影响,旨在提高小鼠的移植效率。方法本研究主要采用输卵管移植技术将2-细胞胚胎移植至假孕鼠体内,对妊娠母鼠的产仔数量进行统计分析。结果与封闭群相比,近交系的移植效率较低,平均产仔率为37.5%,封闭群则为45%。免疫缺陷动物对移植的胚胎数量要求较高,每只小鼠单侧移植11~15枚细胞时的产仔率最高,为63.5%;不同类型小鼠的平均产仔率比较:C57BL/6J(49.8%)>KM(45.8%)>ICR(44.1%)>NOD-SCID(35.6%)>BAL B/c-nu(29.3%)。ICR、C57、NOD-SCID三种小鼠移植胚胎数量在11~15枚时,产仔率最高;而KM小鼠移植胚胎数量在16~30枚时,产仔率最高;BALB/c-nu小鼠移植胚胎数量≤10枚时,产仔率最高。结论不同遗传背景的小鼠输卵管移植效率不同,相同遗传背景小鼠移植产仔率随移植胚胎数量的不同而有所变化。Objective Effects of different numbers of embryos transferred in mice of different genetic backgrounds on the litter rate.Method The 2-cell embryos were transplanted into pseudopregnant mice by fallopian tube transfer technique.Then,Statistical analysis was performed on the litter size of pregnant animals.Result Compared with the closed colony,the transplant efficiency of the inbred strain animals is lower,the average litter rate is 37.5%,and the closed colony is 45%.Immunodeficient animals have higher requirements for the number of embryos to be transplanted,and mice with 11-15 cells have the highest litter rate,63.5%;In this study,mice with different genetic backgrounds have different mean litter rate:C57 BL/6 J(49.8%)>KM(45.8%)>ICR(44.1%)>NOD-SCID(35.6%);>BAL B/c-nu(29.3%).In this study,it was found that mice of different genetic backgrounds required different numbers of embryos to achieve higher litter rate.ICR,C57 and NOD-SCID mice need to transfer 11-15 embryos,KM mice need to transfer 16-30 embryos;BALB/c-nu mice need to transfer≤10 embryos.Conclusion Tubal transplantation differs in mice with different genetic backgrounds.The litter rate of mice of the same genetic background varies with the number of transferred embryos.

关 键 词：封闭群动物 近交系动物 免疫缺陷动物 胚胎数量 产仔率 

分 类 号：Q95-3[生物学—动物学]