玉米Trihelix转录因子家族的筛选与生物信息学分析  

Screening and Bioinformatics Analysis of Maize Trihelix Transcription Factor Family

作　　者：焦鹏 邹俊岩 蒋振忠 曲静[3] 马义勇[3] 关淑艳[3] JIAO Peng;ZOU Junyan;JIANG Zhenzhong;QU Jing;MA Yiyong;GUAN Shuyan(College of Life Sciences,Jilin Agricultural University,Changchun 130118,China;Zhenlai County Plant Protection and Quarantine Station,Baicheng 137300,China;College of Agronomy,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学生命科学学院,长春130118 [2]镇赉县植物保护与植物检疫工作站,白城137300 [3]吉林农业大学农学院,长春130118

出　　处：《吉林农业大学学报》2022年第5期520-527,共8页Journal of Jilin Agricultural University

基　　金：吉林省自然科学基金项目(20190201168JC);吉林省科技引导计划项目(2019080212ZG);吉林省科技支撑计划项目(20180201029NY);吉林省重点攻关项目(20170204005NY)。

摘　　要：Trihelix基因家族是一类调节植物逆境胁迫应答和改良作物品质过程中关键的小型转录因子家族。采用生物信息学方法从玉米全基因组数据库中筛选出43个Trihelix家族成员,为进一步探讨玉米Trihelix转录因子家族的功能,分别进行染色体定位分析、信号肽分析、亚细胞定位分析、磷酸化位点分析、系统进化树构建、蛋白质结构分析和保守基序分析。结果表明:ZmTrihelix家族成员不均匀地分布在1~10号染色体上;该蛋白家族仅有ZmTrihelix1存在信号肽;该蛋白家族83.7%定位在细胞核上,16.3%定位在叶绿体、细胞质膜和线粒体上;该蛋白家族磷酸化主要集中在酪氨酸、丝氨酸和苏氨酸残基上;该家族分为5个亚家族,分别为GT-1、GT-2、GTγ、SIP1和SH4;这些家族成员高度保守,每个亚家族均具有其特殊的结构域;该蛋白家族由多个螺旋、延伸链及转角结构组成。上述研究为探索玉米Trihelix家族成员的功能奠定了基础,进一步为玉米的品质改良和分子育种提供理论参考。Trihelix gene family is a kind of small transcription factor family that is key in regulating plant stress response and improving crop quality.In this study,43 members of the Trihelix family were selected from the whole genome database of maize by bioinformatics methods.In order to further explore the function of the Trihelix transcription factor family of maize,chromosome localization analysis,signal peptide analysis,subcellular localization analysis,phosphorylation site analysis,phylogenetic tree construction,protein structure analysis and conservative motif analysis were carried out respectively.The results showed that the members of ZmTrihelix family are unevenly distributed on chromosome 1-10.Only ZmTrihelix1 signal peptide exists in this protein family;83.7%of the protein family is located on the nucleus,16.3%on chloroplast,cytoplasmic membrane and mitochondria.Phosphorylation of the protein family is mainly concentrated on oxalic acid,serine and threonine residues.The family is divided into five subfamilies,namely GT-1,GT-2,GTγ,SIP1 and SH4;The members of these families are highly conservative,and each subfamily has its special domain.The protein family consists of multiple helix,extended chain and corner structures.The research has laid a foundation for exploring the functions of the members of maize Trihelix family and further provided reference for quality improvement and molecular breeding of maize.

关 键 词：玉米 Trihelix转录因子 生物信息学分析 

分 类 号：S513[农业科学—作物学]