预处理及木质素酶对玉米秸秆酶解糖化效果影响  

Effects of Pretreatment and Ligninase on Enzymatic Hydrolysis and Saccharification of Corn Stalk

作　　者：赵文萱 焦宏官 郑亚强 ZHAO Wenxuan;JIAO Hongguan;ZHENG Yaqiang(College of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学药学院,贵阳550025

出　　处：《吉林农业大学学报》2022年第5期548-556,共9页Journal of Jilin Agricultural University

基　　金：贵州省科技支撑项目(黔科合支撑[2021]203)。

摘　　要：以玉米秸秆为原料,以酶解糖化分析、扫描电镜及傅里叶红外光谱验证为检测指标,建立了高效玉米秸秆预处理方法及条件,并进一步探讨了酶解糖化过程中各木质素酶与纤维素酶、木聚糖酶的协同作用效果及酶解糖化过程中各木质纤维素酶的最佳添加比例及添加量。结果表明:2%CaCO_(3)+1%H_(2)O_(2)在料液比1∶11,温度120℃,时间70 min条件下,可较好地去除木质素并保留纤维素及半纤维素组分;酶解糖化试验表明,木质素酶中漆酶对预处理玉米秸秆水解起主要促进作用,各木质酶的最优添加量分别为漆酶8 U/g,木素过氧化物酶10 U/g,锰过氧化物酶6 U/g,此条件下水解液中的混合糖含量为116 mmol/L,比未添加木质素酶含量提高了16%。试验为低酶用量、高糖得率的高效木质纤维素降解复合酶体系的建立及玉米秸秆木质纤维素原料的高效转化利用奠定了基础。Corn stalk was selected as the research material,enzymatic hydrolysis and saccharification analysis,Scanning Electron Microscopy and Fourier Transform Infrared Spectroscopy were used as detection indexes,and the high-efficiency pretreatment method and conditions of corn straw were established.The synergistic effects of ligninase,cellulase and xylanase in enzymatic hydrolysis and saccharification were studied,and the optimal addition ratio and amount of lignocellulase in the process of enzymatic hydrolysis and saccharification were also discussed.The results showed that 2%CaCO_(3)+1%H_(2)O_(2) could remove lignin and retain cellulose and hemicelluloses well at ratio of 1∶11,temperature of 120℃,and time of 70 min.Enzymatic hydrolysis saccharification experiment showed that laccase in ligninase played a major role in promoting the hydrolysis of pretreated corn straw,and the optimal addition amount of each ligninase was laccase 8 U/g,lignin peroxidase 10 U/g and manganese peroxidase 6 U/g,respectively.Under these conditions,the mixed sugar content in the hydrolyzate was 116 mmol/L,which was 16%higher than that without ligninase added.This study lays foundation for the establishment of a high-efficiency lignocellulose-degrading complex enzyme system with low enzyme dosage and high sugar yield and the efficient conversion and utilization of corn stalk lignocellulose raw materials.

关 键 词：木质素酶 玉米秸秆 木质纤维素 酶解糖化 预处理工艺 

分 类 号：S216.2[农业科学—农业机械化工程]