基于COI条形码序列的梅花鹿鹿皮及其伪品的DNA分子鉴定  

DNA Molecular Identification of Sika Deer Skin and Its Adulterants Based on COI Barcode Sequence

作　　者：都帅 王琦[1] 常天赢 嵩之松 张辉[4] 李庆杰[2] 王全凯[1] DU Shuai;WANG Qi;CHANG Tianying;SONG Zhisong;ZHANG Hui;LI Qingjie;WANG Quankai(College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,China;Research Center of Traditional Chinese Medicine,Affiliated Hospital of Changchun University of Traditional Chinese Medicine,Changchun 130021,China;Changchun Shilu Industry Group Co.,Ltd.,Changchun 130606,China;Changchun University of Traditional Chinese Medicine,Chang-chun 130117,China)

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]长春中医药大学附属医院中医药研究中心,长春130021 [3]长春世鹿鹿业集团有限公司,长春130606 [4]长春中医药大学,长春130117

出　　处：《吉林农业大学学报》2022年第5期595-600,共6页Journal of Jilin Agricultural University

基　　金：吉林省科技创新与科技成果转化计划项目(20150311049YY);吉林省重大科技成果转化项目(20170301003YY);吉林省科技发展计划项目(201603084YY);长春市重大科技成果转化项目(16SS22)。

摘　　要：基于COI条形码序列对满族医药鹿皮胶的正品原料梅花鹿鹿皮及其伪品(将马鹿,赤鹿,黇鹿等与梅花鹿相近鹿种作为伪品)进行DNA分子鉴定,建立NJ树分析梅花鹿鹿皮正品与伪品的区别,以期建立一种快速有效的真伪鉴别方法。结果显示:NJ树中,正品梅花鹿鹿皮样品全部聚集为独立的一支,支持率为100%,有明显的单系性;伪品样品也分别聚集为单独的一支,支持率为100%。基于COI序列的DNA条形码技术,对比基因库,可以有效鉴别梅花鹿鹿皮的真伪,为满族医药传统药材梅花鹿鹿皮的真伪鉴别提供了新的科学方法。Based on COI barcode sequence,DNA molecular identification was carried out on the genuine raw materials of Manchu medical deer skin gum,sika deer skin and its adulterants(red deer,deer and other deer species similar to sika deer are regarded as adulterants),and NJ tree was established to analyze the difference between the genuine sika deer skin and the adulterated sika deer skin,so as to establish a fast and effective identification method of authenticity.The results showed that in the NJ tree,all the samples of the genuine sika deer skin were clustered into an independent branch,with a 100%support rate and obvious unilinearity.The adulterated samples were also aggregated into a single branch,with a support rate of 100%.This study proves that DNA barcode technology based on COI sequence can effectively identify the genuineness of sika deer skin,which provides a new scientific method for authenticity identification of sika deer skin,a traditional Manchu medicinal material.

关 键 词：梅花鹿鹿皮 满族医药 COI序列 伪品 

分 类 号：S825[农业科学—畜牧学]