可视化铁离子荧光探针的合成及性能研究  

Synthesis and properties of visual Fe^(3+) probe

作　　者：余亮 江薇 江蓉[2] 黄良芳[2] 胡晓倩[3] 方辉平 柯仲成[2,4] 史建俊[2] 李长江[2,4] YU Liang;JIANG Wei;JIANG Rong;HUANG Liangfang;HU Xiaoqian;FANG Huiping;KE Zhongcheng;SHI Jianjun;LI Changjiang(Department of medicine,Huangshan vocational and Technical College,Huangshan 245041,China;School of Chemistry and Chemical Engineering,Huangshan University,Huangshan 245041,China;College of Life and Environment Sciences,Huangshan University,Huangshan 245041,China;Research Center of Chinese Medicine Efficacy and Health Technology,Huangshan University,Huangshan 245041,China)

机构地区：[1]黄山职业技术学院医学系,安徽黄山245041 [2]黄山学院化学化工学院,安徽黄山245041 [3]黄山学院生命与环境科学学院,安徽黄山245041 [4]黄山学院中药功效成分与健康技术研究中心,安徽黄山245041

出　　处：《食品与发酵工业》2022年第24期266-272,I0013,I0014,共9页Food and Fermentation Industries

基　　金：安徽省自然科学研究项目(2008085MH269);安徽省重点研究及开发计划项目(202204c06020046);安徽省高校自然科学项目(KJ2021ZD0123;KJ2021A1045;KJHS2017B08);安徽省质量工程项目(2020sjjd120;2021sx154);安徽省大学生创新训练项目(S202110375022)。

摘　　要：铁元素是人体必需的微量元素,与人体健康关系紧密。该研究设计并合成了以罗丹明101、乙二胺和8-羟基久洛尼定为原料的化合物R,对其结构进行表征,并对其探针性能和反应机理进行研究。结果表明,R对Fe^(3+)具有很好的响应,伴随溶液颜色发生明显变化的同时,荧光也发生变化。通过等摩尔连续变化法,得出R与Fe^(3+)的反应络合比为2∶1。R对Fe^(3+)检测限分别为49.2 nmol/L(分光光度法)和135.7 nmol/L(荧光光谱法),均远低于国家饮用水标准值。此外,细胞成像实验表明,R具有良好的生物相容性。因此R可以作为一种新型的视觉荧光探针,用于检测Fe^(3+)含量。Iron is an essential trace element for human body,which is closely related to human health.In this study,compound R was designed and synthesized with rhodamine 101,ethylenediamine and 8-hydroxy-dulonidine as raw materials.Its structure was characterized,and its probe performance and reaction mechanism were studied.The results showed that R had a good response to Fe^(3+),and the fluorescence also changed with the obvious change of solution color.The reaction ratio of R and Fe^(3+)was 2∶1 by equimolar continuous change method.The detection limits of R for Fe^(3+)were 49.2 nmol/L(UV)and 135.7 nmol/L(fluorescence),which were far lower than the Chinese national standard for drinking water.In addition,cell imaging experiments showed that R had good biocompatibility.Therefore,R can be used as a new visual fluorescence probe to detect the content of Fe^(3+).

关 键 词：合成 性能 反应机理 视觉荧光探针 Fe^(3+) 

分 类 号：O657.3[理学—分析化学]