不同上升流速提升对反硝化颗粒污泥反应器的影响  

Effect of Different Upflow Velocity Promotion Strategy on the Performance of Denitrifiying Granular Sludge Reactor

作　　者：季飞 边永欢 赵峰 王振毅 赵大密 李志燕 廉静 JI Fei;BIAN Yong-huan;ZHAO Feng;WANG Zhen-yi;ZHAO Da-mi;LI Zhi-yan;LIAN Jing(Key Laboratory of Pollution Prevention Biotechnology of Hebei Province,School of Environmental Science and Engineering,Hebei University of Science and Technology,Shijiazhuang 050018,China;Key Laboratory of Geological Resources and Environment Monitoring and Protection of Hebei Province,Hebei Institute of Geological Environment Monitoring,Shijiazhuang 052460,China)

机构地区：[1]河北科技大学环境科学与工程学院河北省污染防治生物技术重点实验室,河北石家庄050018 [2]河北省地质环境监测院河北省地质资源环境监测与保护重点实验室,河北石家庄052460

出　　处：《中国给水排水》2022年第23期7-16,共10页China Water & Wastewater

基　　金：国家自然科学基金资助项目(51678387);河北省人才工程资助项目(A201902019)。

摘　　要：为了探究上升流速的不同变化幅度对反硝化颗粒污泥反应器的影响,分别构建了快速提升反应器(以大幅度提升上升流速,记作RH)和慢速提升反应器(以小幅度提升上升流速,记作RL),考察不同上升流速提升策略对反应器性能、反硝化颗粒污泥特性及菌群的影响。结果表明,在RH和RL的上升流速由0.25 m/h提升至0.41 m/h的过程中,NO3--N和COD平均去除率均在92%以上,NO2--N平均积累量为1.5 mg/L左右;当两反应器上升流速进一步提升至0.49 m/h时,RH反应器污泥发生流失,COD去除率在运行的前5 d下降为73.1%左右,NO2--N平均积累量达到16.3 mg/L,粒径<1.4 mm的颗粒占比从34%上升为51.8%;而RL的NO3--N和COD平均去除率和NO2--N平均积累量均变化较小。同时,两反应器颗粒污泥的紧密结合型胞外聚合物(TB-EPS)在此阶段出现不同的变化趋势,其中RL中污泥TB-EPS含量从(32.4±0.8)mg/g增加为(56.2±0.4)mg/g,而RH从(47.1±0.8)mg/g下降为(28.9±0.9)mg/g,表明TB-EPS对于反硝化颗粒污泥维持较为稳定的结构起到了重要作用。高通量测序显示,门水平上利于反硝化的Firmicutes和Planctomycetes在RL反应器中得到富集,而在RH反应器中均出现下降;属水平上主要参与反硝化的Hyphomicrobium占比在RH中明显下降,造成了两反应器反硝化性能稳定性的差异。因此,在所考察的上升流速范围内,RL的上升流速提升策略更有利于反硝化颗粒污泥承受较高的上升流速,并保持反应器的稳定运行。In order to explore the influence of different upflow velocity amplitudes on the denitrification granular sludge(DGS)reactor,both rapid rise reactor(increasing the upflow velocity with a high rate,denoted as RH)and slow rise reactor(increasing the upflow velocity with a low rate,denoted as RL)was constructed.The impact of different upflow velocity promotion strategies on the performance of the reactor was investigated,and the characteristics of the DGS and the bacterial flora was analyzed.The results showed that when the upflow velocity of the RH and RL reactors was increased from 0.25 m/h to 0.41 m/h,the average removal rates of NO3--N and COD were over 92%,the average accumulation of NO2--N was about 1.5 mg/L;when the upflow velocity of the RH and RL reactors was further increased to 0.49 m/h,the sludge in RH ran off,the COD removal rate dropped to about 73.1%in the first 5 days of operation,the average accumulation of NO2--N reached 16.3 mg/L,and the diameter of particles less than 1.4 mm increased from 34%to 51.8%;the average removal rate of NO3--N and COD and the average accumulation of NO2--N in the RL had little change.Meanwhile,the TB-EPS of granular sludge in the two reactors had different variation trend at this stage,the TB-EPS content of the sludge in the RL reactor increased from(32.4±0.8)mg/g to(56.2±0.4)mg/g,while that in RH decreased from(47.1±0.8)mg/g to(28.9±0.9)mg/g.TB-EPS played an important role in maintaining a relatively stable structure of DGS.High-throughput sequencing showed that Firmicutes and Planctomycetes,which were conducive to denitrification at the phylum level,were enriched in the RL reactor,but decreased in the RH reactor;the proportion of Hyphomicrobium,which was mainly involved in denitrification at the genus level,decreased significantly in the RH.The different variation of the microbial community in the two reactors caused the difference in the stability of the denitrification performance.Therefore,within the range of upflow velocity investigated in this study,the upflow

关 键 词：上升流速 反硝化颗粒污泥 胞外聚合物 菌群结构 

分 类 号：TU992[建筑科学—市政工程]