白细胞介素9促进日本血吸虫感染小鼠肝星状细胞激活  

Interleukin-9 promotes the activation of hepatic stellate cells in mice infected with Schistosoma japonicum

作　　者：战廷正[1] 马会会 李青 唐莉莉[1] 何姗姗[1] 唐泽丽 夏超明 ZHAN Ting-zheng;MA Hui-hui;LI Qing;TANG Li-li;HE Shan-shan;TANG Ze-li;XIA Chao-ming(Department of Parasitology,Guangxi Medical University,Nanning,Guangxi 530021,China;Department of Pathogenic Biology,Medical College,Soochow University,Suzhou,Jiangsu 215000,China;Department of Cell Biology,Guangxi Medical University,Chi)

机构地区：[1]广西医科大学寄生虫学教研室,广西南宁530021 [2]苏州大学医学部病原生物学系,江苏苏州215000 [3]广西医科大学细胞生物学教研室

出　　处：《中国血吸虫病防治杂志》2022年第5期514-517,共4页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金(81860358);广西壮族自治区自然科学基金(2018GXNSFAA281059)。

摘　　要：目的 探讨小鼠感染日本血吸虫后,白细胞介素-9(interleukin-9, IL-9)在小鼠肝星状细胞活化中的作用。方法采用肝脏原位灌注消化结合密度梯度离心法,分离日本血吸虫感染7周后小鼠原代肝星状细胞(hepatic stellate cells, HSCs)并进行体外培养。将HSCs分为PBS对照组和IL-9刺激组(浓度20 ng/mL)。分别于刺激后48、72 h收集细胞,采用免疫印迹试验检测HSCs中α平滑肌肌动蛋白(α-smooth muscle actin, α-SMA)、Ⅰ型胶原蛋白(typeⅠcollagen,ColⅠ)和Ⅲ型胶原蛋白(typeⅢcollagen, ColⅢ)表达水平。结果 经20 ng/mL IL-9刺激48 h后,IL-9刺激组HSCs中α-SMA [(0.87±0.02)vs.(0.69±0.01);t=17.39,P <0.01]、ColⅠ[(0.74±0.02)vs.(0.65±0.01);t=9.56,P <0.01]、ColⅢ蛋白[(0.94±0.04)vs.(0.75±0.03);t=6.15,P <0.01]表达水平均显著高于PBS对照组;刺激72 h后,IL-9刺激组HSCs中α-SMA蛋白水平显著高于PBS对照组[(0.76±0.02)vs.(0.58±0.02);t=12.52,P <0.01],但两组间ColⅠ[(0.68±0.02)vs.(0.66±0.02);t=1.15,P> 0.05]和ColⅢ蛋白[(0.75±0.01)vs.(0.72±0.02);t=2.22,P> 0.05]表达水平差异均无统计学意义。结论 IL-9可促进日本血吸虫感染小鼠HSCs激活。Objective To investigate the effects of interleukin-9(IL-9) in the activation of hepatic stellate cells(HSCs) in mice infected with Schistosoma japonicum.Methods Primary HSCs were isolated from mice 7 weeks post-infection with S. japonicum using the in situ liver perfusion and density gradient centrifugation, and cultured in vitro. HSCs were randomly assigned to the PBS control group and IL-9 stimulation group(stimulation with 20 ng/mL IL-9). HSCs were harvested 48 h and 72 h poststimulation, and the expression of α-smooth muscle actin(α-SMA), typeⅠcollagen(ColⅠ) and type Ⅲ collagen(Col Ⅲ) was determined in HSCs using Western blotting.Results Following stimulation with 20 ng/mL IL-9 for 48 h, the expression of α-SMA[(0.87 ± 0.02) vs.(0.69 ± 0.01);t = 17.39, P < 0.01], ColⅠ[(0.74 ± 0.02) vs.(0.65 ± 0.01);t = 9.56, P < 0.01] and Col Ⅲ [(0.94 ±0.04) vs.(0.75 ± 0.03);t = 6.15, P < 0.01] was significantly greater in HSCs in the IL-9 stimulation group than in the PBS control group. Following stimulation with 20 ng/mL IL-9 for 72 h, the expression of α-SMA was significantly greater in HSCs in the IL-9stimulation group than in the PBS control group [(0.76 ± 0.02) vs.(0.58 ± 0.02);t = 12.52, P < 0.01];however, there were no significant differences between the two groups in terms of ColⅠ[(0.68 ± 0.02) vs.(0.66 ± 0.02);t = 1.15, P > 0.05] or Col Ⅲ expression [(0.75 ± 0.01) vs.(0.72 ± 0.02);t = 2.22, P > 0.05].Conclusion IL-9 promotes the activation of HSCs in mice infected with S. japonicum.

关 键 词：日本血吸虫 白细胞介素9 肝星状细胞 肝纤维化 

分 类 号：R383.24[医药卫生—医学寄生虫学]