Recent advances in the use of the CRISPR-Cas system for the detection of infectious pathogens  

作　　者：Hongdan GAO Zifang SHANG Siew Yin CHAN Dongli MA 

机构地区：[1]Institute of Pediatrics,Shenzhen Children's Hospital,Shenzhen 518026,China [2]CAS Key Laboratory of Pathogenic Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China [3]Frontiers Science Center for Flexible Electronics(FSCFE),Xi'an Institute of Flexible Electronics(IFE)&Xi'an Institute of Biomedical Materials and Engineering(IBME),Northwestern Polytechnical University(NPU),Xi'an 710072,China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2022年第11期881-898,共18页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：the Natural Science Basic Research Plan in Shaanxi Province of China(No.2020JQ-148);the Guangdong Basic and Applied Basic Research Foundation(No.2021A1515110793);the Shenzhen Pathogen High-Throughput Sequencing Technical Engineering Laboratory Upgrading Project(No.2019-986),China。

摘　　要：Infectious diseases cause great economic loss and individual and even social anguish.Existing detection methods lack sensitivity and specificity,have a poor turnaround time,and are dependent on expensive equipment.In recent years,the clustered regularly interspaced short palindromic repeats(CRISPR)-CRISPR-associated protein(Cas)system has been widely used in the detection of pathogens that cause infectious diseases owing to its high specificity,sensitivity,and speed,and good accessibility.In this review,we discuss the discovery and development of the CRISPR-Cas system,summarize related analysis and interpretation methods,and discuss the existing applications of CRISPR-based detection of infectious pathogens using Cas proteins.We conclude the challenges and prospects of the CRISPR-Cas system in the detection of pathogens.

关 键 词：CRISPR-Cas DIAGNOSIS Disease detection Infectious disease PATHOGEN 

分 类 号：R440[医药卫生—诊断学] Q78[医药卫生—临床医学]